CAJ | 학술논문

背景：以不同修饰剂制备的纳米钛酸钙具有良好的骨传导性、生物相容性及生物活性。 　　目的：观察纳米钛酸钙材料对成骨细胞增殖、分化的影响。 　　方法：分别以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备纳米钛酸钙，同时制备无修饰剂的纳米钛酸钙。将制备的4组纳米钛酸钙分别配制为0.1，1.0，10 g/L的浸提液，MTT方法检测材料浸提液对乳鼠成骨细胞增殖及碱性磷酸酶活性的影响，以正常培养的细胞为对照。 　　结果与结论：以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备的纳米钛酸钙，无修饰剂的纳米钛酸钙在1.0，10 g/L质量浓度下可明显促进成骨细胞的增殖；上述4组纳米钛酸钙浸提液在1.0，10 g/L质量浓度下，培养3，6，9 d的细胞A值也高于对照组(P<0.05，P<0.01)，培养6，9 d的细胞碱性磷酸酶活性值均高于对照组(P<0.05，P<0.01)。表明以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠、无修饰剂制备的钛酸钙对成骨细胞无毒性作用，可促进成骨细胞的增殖和分化。
배경：이불동수식제제비적납미태산개구유량호적골전도성、생물상용성급생물활성。 　　목적：관찰납미태산개재료대성골세포증식、분화적영향。 　　방법：분별이취을이순、십륙완기삼갑기추화안、저몽산납위수식제제비납미태산개，동시제비무수식제적납미태산개。장제비적4조납미태산개분별배제위0.1，1.0，10 g/L적침제액，MTT방법검측재료침제액대유서성골세포증식급감성린산매활성적영향，이정상배양적세포위대조。 　　결과여결론：이취을이순、십륙완기삼갑기추화안、저몽산납위수식제제비적납미태산개，무수식제적납미태산개재1.0，10 g/L질량농도하가명현촉진성골세포적증식；상술4조납미태산개침제액재1.0，10 g/L질량농도하，배양3，6，9 d적세포A치야고우대조조(P<0.05，P<0.01)，배양6，9 d적세포감성린산매활성치균고우대조조(P<0.05，P<0.01)。표명이취을이순、십륙완기삼갑기추화안、저몽산납、무수식제제비적태산개대성골세포무독성작용，가촉진성골세포적증식화분화。
BACKGROUND:Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility. OBJECTIVE:To observe the influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts. METHODS:Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. Al the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast. RESULTS AND CONCLUSION:The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L) for 3, 6, 9 days exceeded the contrast (P<0.05, P<0.01), as wel as the alkaline phosphatase activity (P<0.05, P<0.01). Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.