CAJ | 학술논문

背景：如何用药物去抑制神经修复后瘢痕的生长成为周围神经损伤后功能恢复的关键。课题组以往研究借鉴广泛应用于抗肿瘤药物局部释放的纳米微球缓释技术设计了一种强的松龙纳米微球缓释膜，取得了良好的体外药物缓释效果。 　　目的：制备强的松龙纳米微球缓释膜，观察该膜的生物相容性及安全性。 　　方法：采用反胶束乳化溶剂挥发法和球膜结合的方法制备强的松龙纳米微球缓释膜，用细胞毒性实验、溶血实验、急慢性全身毒性实验对药膜的生物安全性进行初步评价。 　　结果与结论：培养第7天，L929小鼠成纤维细胞相对增殖率为92.6%，证实此膜无细胞毒性；该膜对新鲜的抗凝兔血溶血率为0.59%，无明显的溶血作用；此缓释膜的浸提液腹腔注射小鼠未见明显的生物学行为的异常，对大鼠肝肾功能无明显影响。结果证实，强的松龙纳米微球缓释膜具有良好的生物相容性，安全无毒性。
배경：여하용약물거억제신경수복후반흔적생장성위주위신경손상후공능회복적관건。과제조이왕연구차감엄범응용우항종류약물국부석방적납미미구완석기술설계료일충강적송룡납미미구완석막，취득료량호적체외약물완석효과。 　　목적：제비강적송룡납미미구완석막，관찰해막적생물상용성급안전성。 　　방법：채용반효속유화용제휘발법화구막결합적방법제비강적송룡납미미구완석막，용세포독성실험、용혈실험、급만성전신독성실험대약막적생물안전성진행초보평개。 　　결과여결론：배양제7천，L929소서성섬유세포상대증식솔위92.6%，증실차막무세포독성；해막대신선적항응토혈용혈솔위0.59%，무명현적용혈작용；차완석막적침제액복강주사소서미견명현적생물학행위적이상，대대서간신공능무명현영향。결과증실，강적송룡납미미구완석막구유량호적생물상용성，안전무독성。
BACKGROUND:After peripheral nerve injury, to inhibit scar formation by drugs is the key to functional recovery. To reduce the amount of scar formation we designed a prednisolone-loaded film which can sustain drug release and good achievement in in vitro drug release test. OBJECTIVE:To prepare the prednisolone implantable film and investigate its in vivo biocompatibility and safety. METHODS:Prednisolone-loaded nanoparticles were first prepared with reverse micellar emulsion-solvent evaporation method, and the composite film and drug-loaded film were further prepared. Then, we investigated the in vivo biocompatibility of drug-loaded film through celltoxicity test, hemolysis test, acute systemic toxicity test, chronic systemic toxicity test. RESULTS AND CONCLUSION:After cultured for 7 days, the relative growth rate of L929 mouse fibroblasts was 92.6%, showing no cytotoxicity. The hemolysis rate of the film was 0.59%, indicating that the material had no hemolysis action. No abnormal biological behaviors were seen in mice after intraperitoneal injection of film extracts, and there were no changes in liver and renal functions in rats. As il ustrated above, we can safely come to a conclusion that prednisolone-loaded film possesses good biocompatibility and can be safely used in the experiment of reducing the scar at sites of peripheral nerve repair.