CAJ | 학술논문

背景：目前有关镍铬合金烤瓷修复体对龈下菌群影响的研究还较少。 　　目的：探讨镍铬合金烤瓷修复体对龈下菌群构成比的影响。 　　方法：选择因怀疑镍铬合金烤瓷修复体影响健康而要求拆除原修复体的烤瓷牙患者9例(患牙12颗)，于镍铬合金烤瓷烤瓷修复体拆除前、拆除后1个月、拆除后3个月时，采用变性梯度凝胶电泳法检测镍铬合金烤瓷修复体基牙及对侧同名天然牙的龈下菌斑。 　　结果与结论：修复体拆除后1，3个月，患牙龈下菌斑的变性梯度凝胶电泳图谱与拆除前相比均发生了明显变化，而且拆后3个月与拆后1个月相比也发生了较明显变化；此外，选择在修复体拆除前龈下菌斑中经常出现而在拆除后消失或减弱的变性梯度凝胶电泳图像中一些特异性条带作16S rDNA片段的序列分析，结果显示这些特异条带的基因序列分别与啮蚀艾肯菌、直形弯曲菌和隐藏优杆菌有着较高的序列相似度。结果表明镍铬合金烤瓷修复体导致了修复基牙龈下菌群组成的变化，并且导致了部分牙周可疑致病菌构成比增加。
배경：목전유관얼락합금고자수복체대간하균군영향적연구환교소。 　　목적：탐토얼락합금고자수복체대간하균군구성비적영향。 　　방법：선택인부의얼락합금고자수복체영향건강이요구탁제원수복체적고자아환자9례(환아12과)，우얼락합금고자고자수복체탁제전、탁제후1개월、탁제후3개월시，채용변성제도응효전영법검측얼락합금고자수복체기아급대측동명천연아적간하균반。 　　결과여결론：수복체탁제후1，3개월，환아간하균반적변성제도응효전영도보여탁제전상비균발생료명현변화，이차탁후3개월여탁후1개월상비야발생료교명현변화；차외，선택재수복체탁제전간하균반중경상출현이재탁제후소실혹감약적변성제도응효전영도상중일사특이성조대작16S rDNA편단적서렬분석，결과현시저사특이조대적기인서렬분별여교식애긍균、직형만곡균화은장우간균유착교고적서렬상사도。결과표명얼락합금고자수복체도치료수복기아간하균군조성적변화，병차도치료부분아주가의치병균구성비증가。
BACKGROUND:Currently, there are few reports on the effect of nickel-chromium (Ni-Cr) al oy porcelain-fused-to-metal (PFM) restoration on subgingival flora of abutment. OBJECTIVE:To investigate the effect of the Ni-Cr al oy PFM restoration on subgingival flora ratio of abutment. METHODS:Nine patients (12 teeth) who suspected that Ni-Cr al oy PFM could affect their health and therefore came to hospital to ask for removal of the prosthesis were selected in this study. Their subgingival plaques of abutment were obtained before and 1 month, 3 months after the Ni-Cr al oy PFM restorations were removed, respectively, and the changes of subgingival flora were observed and analyzed by the method of denaturing gradient gel electrophoresis. RESULTS AND CONCLUSION:The images of denaturing gradient gel electrophoresis in subgingival bacteria of experimental group had significant changes at 1 and 3 months after Ni-Cr al oy PFM restorations removed, furthermore, there were significant differences in the images of denaturing gradient gel electrophoresis at 1 and 3 months. In addition, the specific bands were selected from denaturing gradient gel electrophoresis image that appeared before Ni-Cr al oy PFM restorations removed and weakened or disappeared after the removal of restorations, then 16S rDNA sequence in the specific bands were analyzed. The results showed that the gene sequences of these bands were closest related to Eikenel a corrodens, Campylobacter rectus and Eubacterium saphenu. These findings indicated that the Ni-Cr al oy PFM restorations would result in the changes of the proportion of subgingival microflora and increases in the detection rates of some periodontal pathogens.