CAJ | 학술논문

背景：异种骨因其结构及生物特性与人体骨相似，经理化处理后抗原性降低，具有多孔结构，并且来源丰富、可以长期保存等特点，被认为是解决自体骨与异体骨来源不足的有效方法。 　　目的：比较2种不同处理方法处理后去抗原异种松质骨支架材料的各项理化特性。 　　方法：将经过化学方法处理的羊松质骨放入马弗炉中，在1000℃高温下煅烧2 h制备成煅烧骨，将另一组经过化学处理的羊松质骨放入-80℃冰箱中冷冻4周，然后在真空仪器中干燥制备成冻干骨，将经过超纯水冲洗干净的羊松质骨当做对照组。 　　结果与结论：3组样品均保留与人体骨组织类似的多孔三维结构，通过微观观察，它们的框架保存完整，有较小的孔隙(55-650μm)和较高的孔隙率(65%-80%)，煅烧后的支架材料韧性降低而脆性明显增加，冷冻干燥处理后力学性能则下降不大，经过衍射分析仪测定它们的主要成分为羟基磷灰石，但煅烧组中还含有少量的β-磷酸三钙，而能谱分析则证实3组的钙磷含量比均接近人体的钙磷比。结果提示经过这两种方法处理的松质骨与人体骨组织结构相似，其中各主要元素的含量比也与人体接近，而且具有足够小的孔隙和较高的孔隙率，但是煅烧处理对支架材料的力学性能影响比较大，而经过冷冻干燥处理力学性能下降不大但无法完全去除材料中的抗原成分，它们基本能达到骨组织工程支架材料的基本要求。
배경：이충골인기결구급생물특성여인체골상사，경이화처리후항원성강저，구유다공결구，병차래원봉부、가이장기보존등특점，피인위시해결자체골여이체골래원불족적유효방법。 　　목적：비교2충불동처리방법처리후거항원이충송질골지가재료적각항이화특성。 　　방법：장경과화학방법처리적양송질골방입마불로중，재1000℃고온하단소2 h제비성단소골，장령일조경과화학처리적양송질골방입-80℃빙상중냉동4주，연후재진공의기중간조제비성동간골，장경과초순수충세간정적양송질골당주대조조。 　　결과여결론：3조양품균보류여인체골조직유사적다공삼유결구，통과미관관찰，타문적광가보존완정，유교소적공극(55-650μm)화교고적공극솔(65%-80%)，단소후적지가재료인성강저이취성명현증가，냉동간조처리후역학성능칙하강불대，경과연사분석의측정타문적주요성분위간기린회석，단단소조중환함유소량적β-린산삼개，이능보분석칙증실3조적개린함량비균접근인체적개린비。결과제시경과저량충방법처리적송질골여인체골조직결구상사，기중각주요원소적함량비야여인체접근，이차구유족구소적공극화교고적공극솔，단시단소처리대지가재료적역학성능영향비교대，이경과냉동간조처리역학성능하강불대단무법완전거제재료중적항원성분，타문기본능체도골조직공정지가재료적기본요구。
BACKGROUND:Xenogeneic bone structure and biological characteristics are similar to human bone tissue, and the xenogeneic bone has a decreased antigenicity after physicochemical treatment, with a natural porous structure and rich source, and can be kept for a long time, which is considered to be an effective way to solve the shortage of the autogenous bone and al ograft bone. OBJECTIVE:To compare the physical and chemical properties of xenogenic bone materials prepared by two different methods. METHODS:Sheep cancellous bone treated with chemical method was placed into the muffle furnace at 1 000 ℃for 2 hours to prepare calcined bone. Another cancellous bone was placed into an 80 ℃ refrigerator for 4 weeks and then placed into a vacuum instrument to prepare freeze-dried bone. Cancellous bone rinsed with ultra-pure water served as controls. RESULTS AND CONCLUSION: Three groups of samples retained three-dimensional porous structure which similar with human bone tissue under microscopic observation. The framework was stil intact, with a smal pore of 55-650μm and high porosity of 65%-80%. For the calcined bone, the toughness was decreased and the brittleness increased significantly, but the freeze-dried bone had a little changes in the mechanical properties. Through diffraction analysis, hydroxyapatite was the main composition of the three groups. However, there was a smal amount ofβ-tricalcium phosphate in the calcined bone. Spectrum analysis confirmed that calcium and phosphorus content in these three groups were al close to the human body. The results suggest the cancellous bone treated with these two methods is similar to human bone structure, and the major elements are close to the body. In addition, the cancellous bone after processing has enough smal pore and higher porosity. However, calcination process has a more influence on the mechanical property of scaffold materials, and the freeze-dried bone has a little change but the antigen cannot be completely removed that can reach the basic requirements.