CAJ | 학술논문

背景：椎体切除后的重建依然是脊柱外科医生面临的难题，理想的重建材料应该具有良好的成骨作用、可靠的支持性能、低廉的价格和简单的操作步骤。 　　目的：观察兔颈椎缺损模型填充自体松质骨的异体骨笼重建的可行性和效果。 　　方法：切除兔第四颈椎体建立颈椎缺损模型，利用免肱骨皮质环经冷冻干燥等处理制备同种异体皮质骨笼，并以填充松质骨的皮质骨笼、自体髂骨和单纯皮质骨笼3种材料分别重建兔第四颈椎椎体缺损。 　　结果与结论：X 射线、组织病理学和透射电镜观察均表明，填充有松质骨的皮质骨笼组的成骨作用最明显，骨笼与上下椎体实现了骨性融合。血清碱性磷酸酶检测表明，在重建后2，4周时，填充松质骨的皮质骨笼组和自体髂骨移植组的血清碱性磷酸酶含量差异均无显著性意义，但两组的血清碱性磷酸酶含量均高于单纯异体皮质骨笼组(P<0.01)。生物力学实验表明，填充松质骨的皮质骨笼组在重建后1个月时的稳定性高于自体髂骨移植组(P<0.01)。结果证实，兔颈椎缺损模型填充松质骨的异体骨笼重建后效果满意，强于单纯皮质骨笼和自体髂骨的移植效果，生物相容性良好。
배경：추체절제후적중건의연시척주외과의생면림적난제，이상적중건재료응해구유량호적성골작용、가고적지지성능、저렴적개격화간단적조작보취。 　　목적：관찰토경추결손모형전충자체송질골적이체골롱중건적가행성화효과。 　　방법：절제토제사경추체건립경추결손모형，이용면굉골피질배경냉동간조등처리제비동충이체피질골롱，병이전충송질골적피질골롱、자체가골화단순피질골롱3충재료분별중건토제사경추추체결손。 　　결과여결론：X 사선、조직병이학화투사전경관찰균표명，전충유송질골적피질골롱조적성골작용최명현，골롱여상하추체실현료골성융합。혈청감성린산매검측표명，재중건후2，4주시，전충송질골적피질골롱조화자체가골이식조적혈청감성린산매함량차이균무현저성의의，단량조적혈청감성린산매함량균고우단순이체피질골롱조(P<0.01)。생물역학실험표명，전충송질골적피질골롱조재중건후1개월시적은정성고우자체가골이식조(P<0.01)。결과증실，토경추결손모형전충송질골적이체골롱중건후효과만의，강우단순피질골롱화자체가골적이식효과，생물상용성량호。
BACKGROUND: Vertebral reconstruction is stil a chal enge for spinal surgeons. Ideal reconstruction materials should have good osteogenesis ability, reliable support performance, low price and simple operating steps. OBJECTIVE:To investigate the effect and feasibility of al ogenic cortical ring packed with autogenous cancellous bone in the reconstruction of rabbit cervical vertebra defect. METHODS:Cervical vertebra defect models were established by resection of the fourth cervical vertebra in rabbits. Frozen-dried humeral cortical ring of rabbits was made to prepare the al ogenic cortical ring which was packed with autogenous cancellous bone. Then, al ogenic cortical ring packed with autogenous cancellous bone, single al ogenic cortical ring and autogenous iliac bone were used to repair rabbit cervical vertebra defects. RESULTS AND CONCLUSION:The X-ray, histopathological examination, and scanning electron microscope examinations al showed that the bone union was most significant in group reconstructed with al ogenic cortical ring packed with autogenous cancellous bone. The alkaline phosphates activity in serum was higher in groups reconstructed with al ogenic cortical ring packed with autogenous cancellous bone and with autogenous iliac bone than in group reconstructed with only al ogenic cortical ring at 2 and 4 weeks after operation (P<0.01), but there was no difference between the former two groups. The biomechanical examination showed that the stability of al ogenic cortical ring packed with autogenous cancellous bone was higher than that of autogenous iliac bone at 1 month after operation (P<0.01). These findings indicate that the effect of al ogenic cortical ring packed with autogenous cancellous bone in the reconstruction of rabbit cervical vertebra defects is satisfactory, with a good biocompatibility, which is a promising method for the reconstruction of vertebral defects.