CAJ | 학술논문

研究了温度和光生物反应器的光径对微绿球藻(Nannochloropsis oculata Hibberd)生长及细胞内几种营养成分含量的影响。结果表明，温度对微绿球藻的生长有显著影响(P<0.05)，对几种营养成分的含量有极显著影响(P<0.01)，在28℃的培养条件下，细胞生长速度最快，此时蛋白质含量最高，为5.66%，多糖含量最低，仅为1.47%，但最大生物产量却是在30℃的条件下获得，总脂与叶绿素 a 的含量随温度的升高而降低，类胡萝卜素含量随温度升高而增大。光生物反应器的光径对微绿球藻的生长和总脂、可溶性蛋白、色素的含量有极显著影响(P<0.01)，对多糖含量有显著影响(P<0.05)，比生长速率、最高细胞密度和单位体积产量均随光径的增大而减小，单位面积产量随光径的增大而增大；总脂、可溶性蛋白和多糖的含量随光径的增大而降低，叶绿素和类胡萝卜素含量随光径增大而增大，类胡萝卜素含量的增大趋势比叶绿素更明显。
연구료온도화광생물반응기적광경대미록구조(Nannochloropsis oculata Hibberd)생장급세포내궤충영양성분함량적영향。결과표명，온도대미록구조적생장유현저영향(P<0.05)，대궤충영양성분적함량유겁현저영향(P<0.01)，재28℃적배양조건하，세포생장속도최쾌，차시단백질함량최고，위5.66%，다당함량최저，부위1.47%，단최대생물산량각시재30℃적조건하획득，총지여협록소 a 적함량수온도적승고이강저，류호라복소함량수온도승고이증대。광생물반응기적광경대미록구조적생장화총지、가용성단백、색소적함량유겁현저영향(P<0.01)，대다당함량유현저영향(P<0.05)，비생장속솔、최고세포밀도화단위체적산량균수광경적증대이감소，단위면적산량수광경적증대이증대；총지、가용성단백화다당적함량수광경적증대이강저，협록소화류호라복소함량수광경증대이증대，류호라복소함량적증대추세비협록소경명현。
The effect of temperature and optical path of flat plate glass reactors on growth rate and organics con-tents of Nannochloropsis sp. was investigated, using a range of optical paths from 5 to 20 cm. The results showed that the temperature had a significant effect on the growth of Nannochloropsis (P<0.05), and a very significant ef-fect on the contents of mutrients (P<0.01). The cell growth had a highest speed at 28℃, when the content of soluble protein reached the maximum and that of polysaccharides reached the minimum. The biomass yield reached the maximum at 30℃. The contents of lipid and chlorophyll decreased with the increase of temperature, while the con-tent of carotenoid increased with the increase of temperature. The optical path of photobioreactors had a very sig-nificant effect on the growth of Nannochloropsis (P<0.01). Specific growth rate, maximal cell density (107cell/mL) and yield per unit volume (mg /(L·d) ) decreased with increase in optical path. Yield per unit area (g /(m2·d) ) in-creased with increased optical path. The contents of lipid, soluble protein, and polysaccharides decreased with in-creased optical path, but the contents of chlorophyll and carotenoid increased with increased optical path. The in-crease trend of carotenoids was more obvious than that of chlorophyll. In summary, the optimal optical path deter-mines the optimal culture density, as well as yield per unit volume and the productivity of organics. Under our con-ditions the optimal optical path for culturing Nannochloropsis to accumulate lipid, protein and polysaccharide in plate reactors was 5 cm, the optimal optical path for accumulating pigments was 20 cm.