中国临床新医学
中國臨床新醫學
중국림상신의학
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
2014年
5期
407-410
,共4页
Leelakittisin Benjakarn%蒋伟哲%黄兴振
Leelakittisin Benjakarn%蔣偉哲%黃興振
Leelakittisin Benjakarn%장위철%황흥진
滇桂艾纳香%指纹图谱%高效液相色谱%鉴别
滇桂艾納香%指紋圖譜%高效液相色譜%鑒彆
전계애납향%지문도보%고효액상색보%감별
Blumea riparia(BI.) DC.%Chemical fingerprint%High-performance liquid chromatography ( HPLC)%Identification
目的：建立滇桂艾纳香的高效液相色谱（ HPLC）指纹图谱鉴别方法。方法采用高效液相色谱法。色谱条件包括Phenomenex 溍Synergi 4 u Polar-RP 80A色谱柱（250 mm ×4.6 mm，4μm），甲醇-水（用冰乙酸调pH至2.8）流动相系统，梯度洗脱：0～17 min，10％～40％甲醇；18～37 min，40％～60％甲醇，检测波长256 nm，柱温25℃，体积流量1 ml／min。测定12批滇桂艾纳香的指纹图谱，并作相似度比较分析。结果12批药材依法检测得到256 cm的HPLC指纹图谱，确定了11个特征峰构成的滇桂艾纳香指纹图谱，其中2号峰为原儿茶酸，4号峰为绿原酸。12批的相似度＞0.99，说明12批药材性能良好。结论该方法提供滇桂艾纳香的鉴别方法，有助于推广滇桂艾纳香药材的应用以及为中成药的中间体质量控制打下良好的基础。
目的：建立滇桂艾納香的高效液相色譜（ HPLC）指紋圖譜鑒彆方法。方法採用高效液相色譜法。色譜條件包括Phenomenex 溍Synergi 4 u Polar-RP 80A色譜柱（250 mm ×4.6 mm，4μm），甲醇-水（用冰乙痠調pH至2.8）流動相繫統，梯度洗脫：0～17 min，10％～40％甲醇；18～37 min，40％～60％甲醇，檢測波長256 nm，柱溫25℃，體積流量1 ml／min。測定12批滇桂艾納香的指紋圖譜，併作相似度比較分析。結果12批藥材依法檢測得到256 cm的HPLC指紋圖譜，確定瞭11箇特徵峰構成的滇桂艾納香指紋圖譜，其中2號峰為原兒茶痠，4號峰為綠原痠。12批的相似度＞0.99，說明12批藥材性能良好。結論該方法提供滇桂艾納香的鑒彆方法，有助于推廣滇桂艾納香藥材的應用以及為中成藥的中間體質量控製打下良好的基礎。
목적：건립전계애납향적고효액상색보（ HPLC）지문도보감별방법。방법채용고효액상색보법。색보조건포괄Phenomenex 진Synergi 4 u Polar-RP 80A색보주（250 mm ×4.6 mm，4μm），갑순-수（용빙을산조pH지2.8）류동상계통，제도세탈：0～17 min，10％～40％갑순；18～37 min，40％～60％갑순，검측파장256 nm，주온25℃，체적류량1 ml／min。측정12비전계애납향적지문도보，병작상사도비교분석。결과12비약재의법검측득도256 cm적HPLC지문도보，학정료11개특정봉구성적전계애납향지문도보，기중2호봉위원인다산，4호봉위록원산。12비적상사도＞0.99，설명12비약재성능량호。결론해방법제공전계애납향적감별방법，유조우추엄전계애납향약재적응용이급위중성약적중간체질량공제타하량호적기출。
Obje tcive To establish qualitative chemical fingerprint of Blumea riparia ( BI.)DC.by high-performance liquid chromatography (HPLC).Methods Phenomenex?Synergi 4 u Polar-RP 80A(250 mm ×4.6 mm, 4 μm) column was used with 25℃column temperature then gradient elution was performed by the mobile phase con -sisted of methanol-solution of acetic acid in water(pH 2.8) at 256 nm detecting wavelength with 1 ml/min flow rate. Determination of 12 batches of fingerprint chromatogram of Blumea riparia (BI.) DC.were performed, followed by u-sing the computer-aided similarity evaluation system for similarity analysis of chromatograms .Results Medicinal ma-terials composed of 12 different batches were examined by specified method then we figured out its HPLC chemical fingerprint at 256 nm detection wavelength.Peaks in the chemical fingerprint of Blumea riparia ( BI.)DC.were eval-uatied and overall similarity index were analyzed and compared then 11 common peak were confirmed .This chemical fingerprint consist of 11 characteristic peaks including protocatechuic acid ( the second peak )and chlorogenic acid peak(the fourth peak).According to similarity index able to reflect its medicinal properties , our 12 batches′s simi-larity index more than 0.99 so these 12 batches are high quality medicinal materials .Conclusion The novel reliable quantitative chemical fingerprint analysis could be both foundation of quality assurance at industry level and be an im -portant step for further application of Blumea riparia ( BI.) DC.
