CAJ | 학술논문

目的：探讨基因转染CLD1对大肠癌细胞株SW480粘附作用的影响。方法对SW480 CLD1（转染CLD1的SW480细胞株）、SW480 control （转染了pEGFP-C1的SW480细胞株）、SW480细胞株分别进行细胞同质粘附实验和细胞异质粘附实验。结果细胞同质粘附实验结果显示SW480CLD1细胞粘附细胞数量少于SW480 control （ P＝0.000）和SW480细胞（ P＝0.000），SW480 control的粘附细胞数与SW480无差异（ P＝0.376），粘附细胞数顺序为SW480CLD1＜SW480control和SW480。异质粘附实验的结果显示SW480CLD1粘附细胞数多于SW480control（P＝0.000）和SW480（P＝0.000），差异有统计学意义。 SW480control和SW480粘附细胞数无显著差异（P＝0.334）。粘附细胞数顺序为 SW480CLD1＞SW480control和 SW480。结论 CLD1基因转染后可减少SW480细胞之间的同质粘附作用，增加SW480细胞与Ⅳ型胶原的粘附性。
목적：탐토기인전염CLD1대대장암세포주SW480점부작용적영향。방법대SW480 CLD1（전염CLD1적SW480세포주）、SW480 control （전염료pEGFP-C1적SW480세포주）、SW480세포주분별진행세포동질점부실험화세포이질점부실험。결과세포동질점부실험결과현시SW480CLD1세포점부세포수량소우SW480 control （ P＝0.000）화SW480세포（ P＝0.000），SW480 control적점부세포수여SW480무차이（ P＝0.376），점부세포수순서위SW480CLD1＜SW480control화SW480。이질점부실험적결과현시SW480CLD1점부세포수다우SW480control（P＝0.000）화SW480（P＝0.000），차이유통계학의의。 SW480control화SW480점부세포수무현저차이（P＝0.334）。점부세포수순서위 SW480CLD1＞SW480control화 SW480。결론 CLD1기인전염후가감소SW480세포지간적동질점부작용，증가SW480세포여Ⅳ형효원적점부성。
Objective To investigate the adhesive effects of colorectal cancer cells SW 480 by CLD1 gene transfection.Methods Cell homotypic adhesive tests and cell-matrix adhesive tests were performed on SW480CLD1 (SW480 by CLD1 gene transfection),SW480control(SW480 by pEGFP-C1 transfection) and SW480 cells.Results The result of homotypic adhesive tests showed that the number of homotypic adhesive SW 480 CLD1 cells were less than those of SW480 control ( P =0.000 ) and SW480 cells ( P =0.000 ) .No significant difference was detected between SW480control and SW480 cells(P=0.376).The sequence of number of adhesive cell were SW480CLD1 <SW480control and SW480.The result of cell-matrix adhesive tests showed that the number of SW 480CLD1 adhesive cells were more than those of SW480control(P=0.000) and SW480 cells(P=0.000), significant difference was detected.No signifi-cant difference was detected between SW480control and SW480 cells(P=0.334).The sequence of number of adhesive cell were SW480CLD1 >SW480control and SW480.Conclusion The homotypic adhesion was decrease between SW 480 cells by CLD1 gene transfection.The adhesion was increase between SW480 cells and collagen Ⅳmediated by CLD1 gene transfection .