CAJ | 학술논문

目的：探讨心理应激状态下咬肌痛觉敏感增高现象与三叉神经脊束核尾侧亚核（Vc ）内星形胶质细胞活化的关系。方法：健康雄性SD大鼠40只，随机分为：空白对照组和应激3、7、14 d组（n＝10），实验组大鼠经束缚应激相应时间后，采用旷场实验和高架十字迷宫实验观察各组大鼠焦虑、抑郁样情绪；Von Frey丝刺激检测各组大鼠咬肌的机械性痛阈；免疫组化染色和Western-blot检测Vc内星形胶质细胞活化标志物神经胶质酸性蛋白（GFAP）表达的变化。结果：①束缚应激7、14 d组大鼠在旷场中央停留时间和中央活动总路程，以及在高架十字迷宫开放臂中的滞留时间百分比和进入开放臂次数百分比均较空白对照组明显降低（P＜0．05）；②束缚应激7、14 d组大鼠咬肌机械性痛阈均较空白对照组明显降低（P＜0．05）；③束缚应激7、14 d 组大鼠Vc内GFAP的表达阳性率及其蛋白的表达水平均明显高于空白对照组（P＜0．05）。结论：慢性束缚造成的心理应激能导致Vc内星形胶质细胞的活化，可能参与了心理应激导致的咬肌痛觉敏感性的增高。
목적：탐토심리응격상태하교기통각민감증고현상여삼차신경척속핵미측아핵（Vc ）내성형효질세포활화적관계。방법：건강웅성SD대서40지，수궤분위：공백대조조화응격3、7、14 d조（n＝10），실험조대서경속박응격상응시간후，채용광장실험화고가십자미궁실험관찰각조대서초필、억욱양정서；Von Frey사자격검측각조대서교기적궤계성통역；면역조화염색화Western-blot검측Vc내성형효질세포활화표지물신경효질산성단백（GFAP）표체적변화。결과：①속박응격7、14 d조대서재광장중앙정류시간화중앙활동총로정，이급재고가십자미궁개방비중적체류시간백분비화진입개방비차수백분비균교공백대조조명현강저（P＜0．05）；②속박응격7、14 d조대서교기궤계성통역균교공백대조조명현강저（P＜0．05）；③속박응격7、14 d 조대서Vc내GFAP적표체양성솔급기단백적표체수평균명현고우공백대조조（P＜0．05）。결론：만성속박조성적심리응격능도치Vc내성형효질세포적활화，가능삼여료심리응격도치적교기통각민감성적증고。
AIM:To investigate the role of astrocyte activation in the spinal trigeminal nucleus caudalis (Vc)in the increased masseter mechanical sensitivity induced by psychological stress in rats.METHODS:40 male SD rats were randomly divided into a control group and 3 stress groups (n=10).The rats in stress groups were treated by constraint for 3,7 and 14 days respectively.The stressed condition was testified by open field test and the elevated plus maze test.Von Frey filaments were used to evaluate the mechanical sensitivity of masseter.The expression of glial fibrillary acidic protein (GFAP)was measured by immunohistochemistry and Western-blot.RESULTS:The rats in the 7-and 14-day stress groups showed significant anxious and depressive behavior(P<0.05 ),and increase of masseter mechanical sensitivity(P<0.05).GFAP expression in Vc was increased in the stress groups compared with that in the control group (P<0.05 ).CONCLUSION:Chronic restraint stress can result in astrocytes activation in Vc that may contribute to the increased masseter mechanical sensitivity in rats.