CAJ | 학술논문

目的：评估铜绿假单胞菌-改良 Hodge（PAE-MHT）法对铜绿假单胞菌（PAE）金属β-内酰胺酶（MBLs）检测的临床适用性。方法收集82株临床分离的耐亚胺培南的 PAE，通过双纸片协同法、E-test 法及 PAE-MHT 法作为 MBLs 表型初筛试验，并对几种方法进行比较。聚合酶链反应（PCR）法检测 IMP 型和 VIM 型 MBLs 基因并测序。结果PAE-MHT 法的敏感性、特异性和准确率分别为84．6％，97．2％，97．6％．PAE-MHT 法和双纸片协同法、E-test 法有非常好的一致性。结论PAE-MHT 法简便、快捷，不失为临床快速筛查非发酵菌产 MBLs 的一种新方法。
목적：평고동록가단포균-개량 Hodge（PAE-MHT）법대동록가단포균（PAE）금속β-내선알매（MBLs）검측적림상괄용성。방법수집82주림상분리적내아알배남적 PAE，통과쌍지편협동법、E-test 법급 PAE-MHT 법작위 MBLs 표형초사시험，병대궤충방법진행비교。취합매련반응（PCR）법검측 IMP 형화 VIM 형 MBLs 기인병측서。결과PAE-MHT 법적민감성、특이성화준학솔분별위84．6％，97．2％，97．6％．PAE-MHT 법화쌍지편협동법、E-test 법유비상호적일치성。결론PAE-MHT 법간편、쾌첩，불실위림상쾌속사사비발효균산 MBLs 적일충신방법。
Objective To Evaluate Pseudomonas aeruginosa-Modified Hodge Test(PAE-MHT)for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa (PAE)in the clinical microbiology laboratory.Methods A total of 82 imipenem-resistant PAE clinical isolates was dectected to metallo-β-lactamase(MBLs)by double disk synergy test、E-test and PAE-MHT,and compared with the positive rates of three methods.The IMP、VIM genes were detected by polymerase chain reaction(PCR).Results The sensitivity,specificity and accuracy rate of PAE-MHT is 84.6%, 97.2%,97.6%.Three methods had a good consistency.Conclusion PAE-MHT is a simple,quick test on Pseudomonas for daily use in clinical laboratories.