中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2014年
6期
945-947
,共3页
流式细胞术%血小板相关抗体%ITP%诊断
流式細胞術%血小闆相關抗體%ITP%診斷
류식세포술%혈소판상관항체%ITP%진단
FCM%platelet-associated antibodies%ITP%diagnosis
目的:探讨流式细胞技术检测血小板相关抗体在 ITP 诊断中的意义。方法选择2012年5月至2013年6月在我院血液科住院的30例 ITP 患者为观察组,同期本院健康体检正常者30例为对照组,采用流式细胞技术检测两组对象的外周血血小板相关抗体 PAIg(PAIgG、PAIgM、PAIgA)水平。结果FCM 检测观察组患者血小板抗体, PAIgG 阳性率为70%,平均荧光强度为(25.15±2.61),对照组平均荧光强度为(5.09±0.54),两组比较有显著性差异(P <0.05);观察组 PAIgM 阳性率为80%,平均荧光强度(27.28±2.25),对照组平均荧光强度为(5.18±0.58),两组比较有显著性差异(P <0.05);观察组 PAIgA 阳性率为30%,平均荧光强度为(6.28±0.65),对照组平均荧光强度为(5.98±0.68),两组比较差异无统计学意义(P >0.05)。结论流式细胞仪检测血小板相关抗体在 ITP 诊断中具有重要的临床意义。
目的:探討流式細胞技術檢測血小闆相關抗體在 ITP 診斷中的意義。方法選擇2012年5月至2013年6月在我院血液科住院的30例 ITP 患者為觀察組,同期本院健康體檢正常者30例為對照組,採用流式細胞技術檢測兩組對象的外週血血小闆相關抗體 PAIg(PAIgG、PAIgM、PAIgA)水平。結果FCM 檢測觀察組患者血小闆抗體, PAIgG 暘性率為70%,平均熒光彊度為(25.15±2.61),對照組平均熒光彊度為(5.09±0.54),兩組比較有顯著性差異(P <0.05);觀察組 PAIgM 暘性率為80%,平均熒光彊度(27.28±2.25),對照組平均熒光彊度為(5.18±0.58),兩組比較有顯著性差異(P <0.05);觀察組 PAIgA 暘性率為30%,平均熒光彊度為(6.28±0.65),對照組平均熒光彊度為(5.98±0.68),兩組比較差異無統計學意義(P >0.05)。結論流式細胞儀檢測血小闆相關抗體在 ITP 診斷中具有重要的臨床意義。
목적:탐토류식세포기술검측혈소판상관항체재 ITP 진단중적의의。방법선택2012년5월지2013년6월재아원혈액과주원적30례 ITP 환자위관찰조,동기본원건강체검정상자30례위대조조,채용류식세포기술검측량조대상적외주혈혈소판상관항체 PAIg(PAIgG、PAIgM、PAIgA)수평。결과FCM 검측관찰조환자혈소판항체, PAIgG 양성솔위70%,평균형광강도위(25.15±2.61),대조조평균형광강도위(5.09±0.54),량조비교유현저성차이(P <0.05);관찰조 PAIgM 양성솔위80%,평균형광강도(27.28±2.25),대조조평균형광강도위(5.18±0.58),량조비교유현저성차이(P <0.05);관찰조 PAIgA 양성솔위30%,평균형광강도위(6.28±0.65),대조조평균형광강도위(5.98±0.68),량조비교차이무통계학의의(P >0.05)。결론류식세포의검측혈소판상관항체재 ITP 진단중구유중요적림상의의。
Objective to investigate the significance of platelet-assosiated Ig by flow cytometry (FCM)in the diag-nosis of idiopathic thrombocytopenic pupura.Methods Took the Control study,and selected the types of non- ITP thrombocytopenic patients with various indicators as a reference object FCM was used for detection of platelet associat-ed antibody in 30 patients with ITP and 30 healthy control.Results Using the FCM detection of platelet antibodies in patients with ITP,Positive rate of PAIgG was 70%,the average of fluorescence intensity was(25.15 ± 2.61),and the average of fluorescence intensity in normal control group was (5.09 ± 0.54),there was a significant difference between the two (P <0.05);Positive rate of PAIgA was 80%,the average of fluorescence intensity was(27.28±2.25),and the average of fluorescence intensity in normal control group was(5.18±0.58),there was a significant difference between the two (P <0.05);Positive rate of PAIgG was 30%,the average of fluorescence intensity was 6.28±0.65,and the av-erage of fluorescence intensity in normal control group was 5.98±0.68,there wasn’t a significant difference between the two (P <0.05);Conclusion Flow cytometry detection of platelet-associated antibodies have important significance for the diagnosis of ITP.