CAJ | 학술논문

目的：研究 PKA-RhoA 信号传导通路在 EAE 发病中的作用，观察中药复方益肾达络饮干预实验性自身免疫性脑脊髓炎（EAE）的疗效，探讨益肾达络饮治疗 EAE 的作用机制，以及对 EAE 小鼠中枢神经损伤后神经再生的作用。方法采用 Western blot 方法测定各组小鼠脑和脊髓组织中 PKAC-a、RhoA 的含量，旨在了解 PKAC-a、RhoA 在 EAE 发生后的作用以及益肾达络饮对神经再生的作用情况。结果检测脑组织中 PKAC-a 表达显示，正常组和模型组、中药组、激素组均有差异（P 值均＜0．05）；模型组和抑制剂组、中药组、激素组均有显著性差异（P 值均＜0．05）；中药组和激素组有极显著性差异（P 值＜0．01）。RhoA 根据多项比较组间 P 值可知：脊髓组织中，正常组和模型组、激素组、中药＋激素组、抑制剂组均有差异（P 值均＜0．05）；模型组和抑制剂组有显著性差异（P 值＜0．05），和中药组、激素组、中药＋激素组有极显著性差异（P 值＜0．01）；脑组织中，模型组和中药＋激素组有显著性差异（P ＜0．05），和中药组有极显著性差异（P 值＜0．01）。结论cAMP-PKA-RhoA 信号通路与 p38MAPK 的表达有关，多发性硬化（MS）在中枢神经系统中存在发病部位的区别，中药益肾达络饮和激素醋酸泼尼松在促进中枢神经再生时均有疗效，作用大小不同。
목적：연구 PKA-RhoA 신호전도통로재 EAE 발병중적작용，관찰중약복방익신체락음간예실험성자신면역성뇌척수염（EAE）적료효，탐토익신체락음치료 EAE 적작용궤제，이급대 EAE 소서중추신경손상후신경재생적작용。방법채용 Western blot 방법측정각조소서뇌화척수조직중 PKAC-a、RhoA 적함량，지재료해 PKAC-a、RhoA 재 EAE 발생후적작용이급익신체락음대신경재생적작용정황。결과검측뇌조직중 PKAC-a 표체현시，정상조화모형조、중약조、격소조균유차이（P 치균＜0．05）；모형조화억제제조、중약조、격소조균유현저성차이（P 치균＜0．05）；중약조화격소조유겁현저성차이（P 치＜0．01）。RhoA 근거다항비교조간 P 치가지：척수조직중，정상조화모형조、격소조、중약＋격소조、억제제조균유차이（P 치균＜0．05）；모형조화억제제조유현저성차이（P 치＜0．05），화중약조、격소조、중약＋격소조유겁현저성차이（P 치＜0．01）；뇌조직중，모형조화중약＋격소조유현저성차이（P ＜0．05），화중약조유겁현저성차이（P 치＜0．01）。결론cAMP-PKA-RhoA 신호통로여 p38MAPK 적표체유관，다발성경화（MS）재중추신경계통중존재발병부위적구별，중약익신체락음화격소작산발니송재촉진중추신경재생시균유료효，작용대소불동。
Objective To investigate the effect of PKA-RhoA signal transduction system on experimental autoim-mune encephalomyelitis and investigate the mechanism of Yishen Daluo Decoction,to explore the efficiency of Yishen Daluo Decoction on treating experimental autoimmune encephalomyelitis.Methods Western Blot was used to explore the expression of PKAC-a,RhoA in the CNS of SJL mice.Results Compared with normal group,the content of PKAC-a in brain was reduced in model group and Yishen Daluo Decoction-treated group(P <0.05),and was improved in hor-mone-treated group (P < 0.05 ).Compared with model group,the content of PKAC-a in brain was improved in SB203580-treated group and hormone-treated group(P < 0.05),and was reduced in Yishen Daluo Decoction-treated <br> group(P < 0.05),and it was shown statistically significant differences between the Yishen Daluo Decoction-treated group and hormone-treated group (P <0.01).Compared with normal group,the content of RhoA in spinal cord was im-proved in model group and hormone-treated group and SB203580-treated group(P < 0.05),and was reduced in hor-mone-YiShen Da Luo Decoction complex treated group(P <0.05).Compared with model group,the content of RhoA in spinal cord was improved in hormone-treated group(P <0.05)and SB203580-treated group(P <0.01),and was re-duced in Yishen Daluo Decoction-treated group and hormone- YiShen Da Luo Decoction complex treated group(P <0.05).Compared with model group,the content of RhoA in brain was reduced in hormone-YiShen Da Luo Decoction complex treated group(P <0.05),and was improved in Yishen Daluo Decoction-treated group(P <0.01).Conclusion The results of present study indicate that cAMP-PKA-RhoA signal transduction system is proposed by regulating the expression of p38MAPK.There is difference between the disease sites of multiple sclerosis (MS)in the central nervous system.Yishen Daluo Decoction and Prednisone are both effective in promoting the regeneration of central nervous.