CAJ | 학술논문

目的：研究早期激素性股骨头坏死(ONFH)动物模型的建立方法。方法健康雄性比格犬20只，随机分为对照组(n=10)和实验组(n=10)。实验组肌注大肠杆菌内毒素10μg/kg，然后连续3 d肌注甲基泼尼松龙20 mg/kg；对照组肌注等量生理盐水。两组分别于给药后2个月、4个月进行MRI检查，然后每组各处死5只动物，切取双侧股骨头标本做组织学检查。两组均在注射药物前后24 h抽静脉血化验血浆凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、抗凝血酶-Ⅲ(AT-Ⅲ)。结果实验组给药后2个月病理结果显示，有4髋发生ONFH，MRI未显示异常；给药后4个月病理结果显示，有6髋发生ONFH，MRI未显示异常。实验组注射药物后PT、APTT、AT-Ⅲ值显著缩短(P<0.001)，且显著短于对照组(P<0.001)。结论改良激素法可以建立早期股骨头坏死的动物模型，高凝低纤溶可能是激素性骨坏死的原因。
목적：연구조기격소성고골두배사(ONFH)동물모형적건립방법。방법건강웅성비격견20지，수궤분위대조조(n=10)화실험조(n=10)。실험조기주대장간균내독소10μg/kg，연후련속3 d기주갑기발니송룡20 mg/kg；대조조기주등량생리염수。량조분별우급약후2개월、4개월진행MRI검사，연후매조각처사5지동물，절취쌍측고골두표본주조직학검사。량조균재주사약물전후24 h추정맥혈화험혈장응혈매원시간(PT)、활화부분응혈매시간(APTT)、항응혈매-Ⅲ(AT-Ⅲ)。결과실험조급약후2개월병리결과현시，유4관발생ONFH，MRI미현시이상；급약후4개월병리결과현시，유6관발생ONFH，MRI미현시이상。실험조주사약물후PT、APTT、AT-Ⅲ치현저축단(P<0.001)，차현저단우대조조(P<0.001)。결론개량격소법가이건립조기고골두배사적동물모형，고응저섬용가능시격소성골배사적원인。
Objective To explore the method to establish animal model of early osteonecrosis of the femoral head (ONFH) induced by steroid. Methods 20 healthy male Beagles were randomly divided into control group and experimental group with 10 dogs in each group. The experimental group was injected lipopolysaccharide 10μg/kg and methylprednisolone 20 mg/kg for 3 days consecutively. The control group was injected normal saline. 2 months and 4 months after administration, both groups were performed magnetic resonance imaging (MRI). 5 animals were sacrificed respectively at 2 months and 4 months after administration in each group, and bilateral femoral head speci-mens were obtained to perform histological examination. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), anti-thrombin III (AT-III) were tested before and 24 h after administration. Results In the experimental group, the pathological results showed that there were 4 ONFH 2 months and 6 ONFH 4 months after administration and MRI did not show any abnormality. Compared with the control group, the PT, APTT, AT-III in the experiment group shortened significantly after administration (P<0.001). Conclusion Modified steroid-induced method can establish the animal model of early ONFH. Hypercoagulation and low fibrinolysis may be the reason of ste-roid-induced osteonecrosis.