CAJ | 학술논문

从某橡胶厂的活性污泥中筛选得到一株可高效降解苯乙烯的微生物菌株 WJ,通过透射电镜(TEM)、生理生化试验、Biolog 鉴定和16S rRNA基因的系统发育树建立等分析方法,鉴定该菌株为恶臭假单胞菌(Pseudomonas putida).通过环境单因素试验,确定该菌株WJ在温度30℃、pH值7.0的培养条件下具有良好的降解效果.通过不同初始浓度苯乙烯的降解及生长特性,确定该菌株的最大底物耐受浓度达到1500mg/L,且其生长动力学过程符合Haldane’s方程,相应动力学参数为：vmax=0.282h-1,Ks=23.57mg/L,Ki=1784.56mg/L.底物宽泛性试验表明,该菌株对苯系物(BTEX)、正己烯等与苯乙烯结构类似的碳氢化合物都具有良好的降解能力,表明其具备良好的底物宽泛性.该菌株对苯乙烯的氧化降解途径主要为乙烯基侧链的氧化.
종모상효엄적활성오니중사선득도일주가고효강해분을희적미생물균주 WJ,통과투사전경(TEM)、생리생화시험、Biolog 감정화16S rRNA기인적계통발육수건립등분석방법,감정해균주위악취가단포균(Pseudomonas putida).통과배경단인소시험,학정해균주WJ재온도30℃、pH치7.0적배양조건하구유량호적강해효과.통과불동초시농도분을희적강해급생장특성,학정해균주적최대저물내수농도체도1500mg/L,차기생장동역학과정부합Haldane’s방정,상응동역학삼수위：vmax=0.282h-1,Ks=23.57mg/L,Ki=1784.56mg/L.저물관범성시험표명,해균주대분계물(BTEX)、정기희등여분을희결구유사적탄경화합물도구유량호적강해능력,표명기구비량호적저물관범성.해균주대분을희적양화강해도경주요위을희기측련적양화.
A bacterium (strain WJ) capable of efficiently degrading styrene was isolated from the activated sludge of a rubber plant. According to its morphology, physiological-biochemical properties and its16S rRNA gene sequence analysis, the strain was identified as Pseudomonas putida. The single-factor test showed that the optimum temperature and pH for its growth and styrene-degradation were 30℃ and 7.0, respectively. Strain WJ could efficiently degrade various concentration of styrene, and the maximum tolerated concentration was 1500mg/L. Haldane's model could be fit to the kinetics data well and its degradation kinetics parameters listed as below:vmax=0.282h-1, Ks=23.57mg/L, Ki=1784.56mg/L. The strain WJ could biodegrade several hydrocarbons (such as BTEX, n-hexene), which were characterized with similar structures to that of styrene. Analysis of metabolic products showed that the main degradation pathway of styrene by strain WJ was the oxidation of oxyethylene chain.