CAJ | 학술논문

目的：采用小鼠骨髓细胞微核试验，对脱氢乙酸进行体内致突变安全性评价。方法按照1/10 LD50、1/5 LD50和1/2 LD50设置三个剂量组，另设置阴性对照组和环磷酰胺（ CP）阳性对照组（100 mg·kg-1）。每组10只小鼠，雌雄各半，灌胃给药，两次攻毒时间间隔24 h。在第二次灌胃后6 h，处死小鼠，制片，观察并计数嗜多染红细胞的微核率，并与阳性对照组和隐形对照组进行比较分析。结果1/5 LD50、1/10 LD50剂量时，与正常对照组无显著差异；1/2 LD50剂量组与正常对照组有显著差异（ P﹤0.05），但无剂量-效应关系。结论脱氢乙酸小鼠骨髓细胞微核试验结果为阴性。
목적：채용소서골수세포미핵시험，대탈경을산진행체내치돌변안전성평개。방법안조1/10 LD50、1/5 LD50화1/2 LD50설치삼개제량조，령설치음성대조조화배린선알（ CP）양성대조조（100 mg·kg-1）。매조10지소서，자웅각반，관위급약，량차공독시간간격24 h。재제이차관위후6 h，처사소서，제편，관찰병계수기다염홍세포적미핵솔，병여양성대조조화은형대조조진행비교분석。결과1/5 LD50、1/10 LD50제량시，여정상대조조무현저차이；1/2 LD50제량조여정상대조조유현저차이（ P﹤0.05），단무제량-효응관계。결론탈경을산소서골수세포미핵시험결과위음성。
Objective To investigate mutational function of dehydroacetic acid( DHA). Methods The experimental animals were randomly divided into five groups such as negative control group,1/10 LD50 ,1/5 LD50 ,1/2 LD50 and positive control group,respectively. Ten animals were in each group,half male and female. The mice received DHA through gavage by 30 h method(24 h interval between 2 times). Sliders were made,polychromatic erythrocyte(PCE)was observed and rates of their micromucleus were counted an compared with positive and negative groups. Results Beside of 1/5 LD50 ,1/10 LD50 DHA groups,the 1/2 LD50 group was obviously different from normal mice(P﹤0. 05),but there were not dose-effect relationship within three doses of DHA groups. Conclusion The micronucleus test of DHA were observated a nega-tive result.