实用药物与临床
實用藥物與臨床
실용약물여림상
PRACTICAL PHARMACY AND CLINICAL REMEDIES
2014年
6期
740-743
,共4页
丝裂霉素%角膜%渗透%高效液相色谱%一级动力学
絲裂黴素%角膜%滲透%高效液相色譜%一級動力學
사렬매소%각막%삼투%고효액상색보%일급동역학
Mitomycin%Cornea%Penetration%HPLC%First order kinetics
目的：考察丝裂霉素在兔离体角膜中的渗透特性。方法以谷胱甘肽缓冲液为接收液,使用水平式扩散仪考察丝裂霉素在离体兔角膜中的渗透特性；样品使用HPLC测定,采用Agilent Zorbax C18柱(150 mm ×4．6 mm,5μm),流动相为甲醇∶水(70∶30,v/v),流速为1 mL/min,检测波长为365 nm。结果建立的丝裂霉素HPLC测定方法专属性、线性、精密度等均良好,丝裂霉素在兔角膜中的渗透按照一级动力学拟合时相关系数最高。结论丝裂霉素在兔离体角膜中的渗透符合一级动力学过程。
目的：攷察絲裂黴素在兔離體角膜中的滲透特性。方法以穀胱甘肽緩遲液為接收液,使用水平式擴散儀攷察絲裂黴素在離體兔角膜中的滲透特性；樣品使用HPLC測定,採用Agilent Zorbax C18柱(150 mm ×4．6 mm,5μm),流動相為甲醇∶水(70∶30,v/v),流速為1 mL/min,檢測波長為365 nm。結果建立的絲裂黴素HPLC測定方法專屬性、線性、精密度等均良好,絲裂黴素在兔角膜中的滲透按照一級動力學擬閤時相關繫數最高。結論絲裂黴素在兔離體角膜中的滲透符閤一級動力學過程。
목적：고찰사렬매소재토리체각막중적삼투특성。방법이곡광감태완충액위접수액,사용수평식확산의고찰사렬매소재리체토각막중적삼투특성；양품사용HPLC측정,채용Agilent Zorbax C18주(150 mm ×4．6 mm,5μm),류동상위갑순∶수(70∶30,v/v),류속위1 mL/min,검측파장위365 nm。결과건립적사렬매소HPLC측정방법전속성、선성、정밀도등균량호,사렬매소재토각막중적삼투안조일급동역학의합시상관계수최고。결론사렬매소재토리체각막중적삼투부합일급동역학과정。
Objective To investigate the penetration behavior of mitomycin in isolated cornea of rabbits. Methods The penetration behavior of mitomycin in isolated rabbit cornea was investigated on horizontal diffusion cells using glutathione buffer as receptor medium. The samples were determined by HPLC and separation was achieved on an Agilent Zorbax C18 column. The mobile phase consisted of methanol/water (70/30,v/v) and delivered at a flow rate of 1 mL/min. The detective wavelength was set at 365 nm. Results The established HPLC method had shown good selectivity,linearity and precision. The correlation index was the highest when the penetration profile of mitomycin in isolated rabbit cornea was fitted by first order kinetic equation. Conclusion The penetration behavior of mitomycin in isolated rabbit cornea accords to first order kinetics.
