实用药物与临床
實用藥物與臨床
실용약물여림상
PRACTICAL PHARMACY AND CLINICAL REMEDIES
2014年
6期
738-740
,共3页
土霉素%高效液相色谱法%含量测定
土黴素%高效液相色譜法%含量測定
토매소%고효액상색보법%함량측정
Oxytetracycline%HPLC%Content determination
目的：建立高效液相色谱法测定土霉素片的含量。方法采用Diamonsil C18(2)5μm 250×4．6mm色谱柱,流动相为0．01 mol/L磷酸二氢钠溶液-乙腈=81∶19(用磷酸调pH为2．0),流速0．8 mL/min,检测波长353 nm,柱温30℃。结果土霉素片在浓度5~30μg/mL内与峰面积具有良好的线性关系,平均回收率为99．5%,RSD=1．3%。结论该方法简便、快速、准确。
目的：建立高效液相色譜法測定土黴素片的含量。方法採用Diamonsil C18(2)5μm 250×4．6mm色譜柱,流動相為0．01 mol/L燐痠二氫鈉溶液-乙腈=81∶19(用燐痠調pH為2．0),流速0．8 mL/min,檢測波長353 nm,柱溫30℃。結果土黴素片在濃度5~30μg/mL內與峰麵積具有良好的線性關繫,平均迴收率為99．5%,RSD=1．3%。結論該方法簡便、快速、準確。
목적：건립고효액상색보법측정토매소편적함량。방법채용Diamonsil C18(2)5μm 250×4．6mm색보주,류동상위0．01 mol/L린산이경납용액-을정=81∶19(용린산조pH위2．0),류속0．8 mL/min,검측파장353 nm,주온30℃。결과토매소편재농도5~30μg/mL내여봉면적구유량호적선성관계,평균회수솔위99．5%,RSD=1．3%。결론해방법간편、쾌속、준학。
Objective To establish an HPLC method for determining the content of oxytetracycline tablets. Methods The Diamonsil C18(2) (250 × 4. 6 mm,5 μm) column was used. The mobile phase was 0. 01 mol/L am-monium di-hydrogen phosphate solution-acetonitrile (81:19) (adjust to pH 2. 0 with phosphoric acid). The flow rate was 0. 8 mL /min and the detective wavelength was at 353 nm. The column temperature was 30 ℃. Results Oxytetra-cycline had a good linear relation in the range of 5~30μg/mL and the average recovery was 99. 5%( RSD=1. 3%) . Conclusion The method is accurate,simple and convenient.
