CAJ | 학술논문

通过响应面法对微生物诱导 CaCO3沉积的培养基组分进行优化。结果表明，最适宜培养基配方为葡萄糖30g/L、大豆蛋白胨10g/L、尿素50g/L、硝酸钙0.5mol/L、吐温80体积分数0.05%以及氯化镍250μmol/L。葡萄糖浓度和尿素浓度、葡萄糖浓度和硝酸钙浓度、葡萄糖浓度和吐温浓度、硝酸钙浓度和吐温浓度以及尿素浓度和氯化镍浓度，对微生物诱导CaCO3沉淀量的影响有着比较显著的双因子效应。沉淀物CaCO3含有少量有机质，形成方解石型或方解石和球霰石混合晶型的CaCO3，其形貌和堆积密度随外界条件改变而不同。
통과향응면법대미생물유도 CaCO3침적적배양기조분진행우화。결과표명，최괄의배양기배방위포도당30g/L、대두단백동10g/L、뇨소50g/L、초산개0.5mol/L、토온80체적분수0.05%이급록화얼250μmol/L。포도당농도화뇨소농도、포도당농도화초산개농도、포도당농도화토온농도、초산개농도화토온농도이급뇨소농도화록화얼농도，대미생물유도CaCO3침정량적영향유착비교현저적쌍인자효응。침정물CaCO3함유소량유궤질，형성방해석형혹방해석화구산석혼합정형적CaCO3，기형모화퇴적밀도수외계조건개변이불동。
Response surface methodology was applied to optimize the medium composition for microorganism induced CaCO3 precipitation. The results show that the optimal medium formulation was glucose 30g/L,soybean peptone 10g/L,urea 50g/L,calcium nitrate concentration 0.5mol/L, Tween 80 concentration 0.05%and chlorination nickel 250μmol/L. The glucose concentration and urea concentration,glucose concentration and calcium nitrate concentration,glucose concentration and Tween concentration , calcium nitrate concentration and Tween concentration as well as urea concentration and chlorination nickel concentration had significantly two-factor effect on microorganism induced CaCO3 precipitation yield. Precipitated CaCO3 contained a small amount of organic matter,and the morphology and bulk density of CaCO3 on forming calcite or calcite-vaterite mixed crystal depended on precipitation conditions changes.