国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
9期
806-808
,共3页
加味二妙丸%盐酸小檗碱%高效液相色谱法
加味二妙汍%鹽痠小檗堿%高效液相色譜法
가미이묘환%염산소벽감%고효액상색보법
modified Ermiao pill%Berberine hydrochloride%RP-HPLC
目的 建立测定加味二妙丸中盐酸小檗碱含量的HPLC方法.方法 采用Luna-C18柱(4.6 mm×250 mm,5μm),以乙腈:0.1％磷酸(45:55)为流动相,流速1.0 ml/min,柱温30℃,检测波长245 nm.结果 盐酸小檗碱在0.071～1.42mg/ml浓度范围内有良好线性关系(r=0.9997),平均加样回收率为99.2％,RSD小于2.0％.结论 本方法简便,快速,准确,重复性好,可用于该制剂的质量控制.
目的 建立測定加味二妙汍中鹽痠小檗堿含量的HPLC方法.方法 採用Luna-C18柱(4.6 mm×250 mm,5μm),以乙腈:0.1％燐痠(45:55)為流動相,流速1.0 ml/min,柱溫30℃,檢測波長245 nm.結果 鹽痠小檗堿在0.071～1.42mg/ml濃度範圍內有良好線性關繫(r=0.9997),平均加樣迴收率為99.2％,RSD小于2.0％.結論 本方法簡便,快速,準確,重複性好,可用于該製劑的質量控製.
목적 건립측정가미이묘환중염산소벽감함량적HPLC방법.방법 채용Luna-C18주(4.6 mm×250 mm,5μm),이을정:0.1％린산(45:55)위류동상,류속1.0 ml/min,주온30℃,검측파장245 nm.결과 염산소벽감재0.071～1.42mg/ml농도범위내유량호선성관계(r=0.9997),평균가양회수솔위99.2％,RSD소우2.0％.결론 본방법간편,쾌속,준학,중복성호,가용우해제제적질량공제.
Objective To establish a HPLC method for the determination of berberine hydrochloride in Jiawei Ermiao Wan.Methods The RP-HPLC system consisted of the Luna-C18 (4.6 mm × 250 mm,5 μm) column,the mobile phase consisted of acetonitrile-0.1％H3PO4(45:55),the flow rate was 1.0mL · min-1,the column temperature was 30℃,The UV detector was set at 245nm.Results The linear response range was 0.071～1.42 mg· ml-1 (r=0.9998).The average recovery of mangiferin was 99.2％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of berberine hydrochloride in Jiawei Ermiao Wan.
