中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
6期
640-642
,共3页
江明友%万明发%冯亮%梅金红%洪钟亮
江明友%萬明髮%馮亮%梅金紅%洪鐘亮
강명우%만명발%풍량%매금홍%홍종량
前列腺肿瘤%肿瘤标记,生物学
前列腺腫瘤%腫瘤標記,生物學
전렬선종류%종류표기,생물학
Prostatic neoplasms%Tumor markers,biological
目的 探讨Oct4在前列腺癌中的表达及临床意义. 方法 反转录-聚合酶链反应(RT-PCR)半定量分析2例正常前列腺和8例前列腺癌组织标本中Oct4 mRNA表达,SP免疫组织化学法检测10例正常前列腺、10例前列腺增生(BPH)和45例前列腺癌组织标本中Oct4蛋白表达水平. 结果 8例前列腺癌组织中Oct4 mRNA表达相对值分别为0.91、0.76、0.66、0.63、0.55、0.50、0.48、0.42,2例正常前列腺中相对值分别为0.正常前列腺、BPH和前列腺癌组织中Oct4蛋白阳性表达率分别为0.0％、0.0％和91.1％,前列腺癌组织中Oct4蛋白表达水平高于正常前列腺组织和BPH(P＜0.05).Oct4蛋白表达水平随前列腺癌临床分期、病理分级增高而增强.在有和没有淋巴结转移前列腺癌组织中Oct4强阳性表达率分别为72.7％、30.0％(P＜0.05). 结论 Oct4在前列腺癌组织中表达与分化程度、侵袭性关系密切,提示Oct与前列腺癌恶性程度有关,可能是评估前列腺癌预后的重要生物指标之一.
目的 探討Oct4在前列腺癌中的錶達及臨床意義. 方法 反轉錄-聚閤酶鏈反應(RT-PCR)半定量分析2例正常前列腺和8例前列腺癌組織標本中Oct4 mRNA錶達,SP免疫組織化學法檢測10例正常前列腺、10例前列腺增生(BPH)和45例前列腺癌組織標本中Oct4蛋白錶達水平. 結果 8例前列腺癌組織中Oct4 mRNA錶達相對值分彆為0.91、0.76、0.66、0.63、0.55、0.50、0.48、0.42,2例正常前列腺中相對值分彆為0.正常前列腺、BPH和前列腺癌組織中Oct4蛋白暘性錶達率分彆為0.0％、0.0％和91.1％,前列腺癌組織中Oct4蛋白錶達水平高于正常前列腺組織和BPH(P＜0.05).Oct4蛋白錶達水平隨前列腺癌臨床分期、病理分級增高而增彊.在有和沒有淋巴結轉移前列腺癌組織中Oct4彊暘性錶達率分彆為72.7％、30.0％(P＜0.05). 結論 Oct4在前列腺癌組織中錶達與分化程度、侵襲性關繫密切,提示Oct與前列腺癌噁性程度有關,可能是評估前列腺癌預後的重要生物指標之一.
목적 탐토Oct4재전렬선암중적표체급림상의의. 방법 반전록-취합매련반응(RT-PCR)반정량분석2례정상전렬선화8례전렬선암조직표본중Oct4 mRNA표체,SP면역조직화학법검측10례정상전렬선、10례전렬선증생(BPH)화45례전렬선암조직표본중Oct4단백표체수평. 결과 8례전렬선암조직중Oct4 mRNA표체상대치분별위0.91、0.76、0.66、0.63、0.55、0.50、0.48、0.42,2례정상전렬선중상대치분별위0.정상전렬선、BPH화전렬선암조직중Oct4단백양성표체솔분별위0.0％、0.0％화91.1％,전렬선암조직중Oct4단백표체수평고우정상전렬선조직화BPH(P＜0.05).Oct4단백표체수평수전렬선암림상분기、병리분급증고이증강.재유화몰유림파결전이전렬선암조직중Oct4강양성표체솔분별위72.7％、30.0％(P＜0.05). 결론 Oct4재전렬선암조직중표체여분화정도、침습성관계밀절,제시Oct여전렬선암악성정도유관,가능시평고전렬선암예후적중요생물지표지일.
Objective To study the expression and its clinical significance of Oct4 in prostate cancer.Methods The Oct4 mRNA expression levels in 2 normal prostate (NP) samples and 8 prostate cancer (Pca) samples were determined by reverse transcription polymerase chain reaction (RT-PCR) analysis.The Oct4 protein expression levels in 10 NP samples,10 benign prostate hyperplasia (BPH) samples and 45 Pca samples were detected by immunohisochemical analysis.Results The relative expression levels of Oct4 mRNA were 0.91,0.76,0.66,0.63,0.55,0.50,0.48,0.42 in 8 Pca samples and 0 in 2 NP samples,respectively.The positive rates of Oct4 protein expression was higher in Pca tissues than in NP and BPH tissues (91.1％ vs.0.0％,91.1％ vs.0.0％,both P＜0.05).The level of Oct4 protein was increased with higher hostologic grade and clinical stage of prostate cancer.The strong positive rates of Oct4 expression were 72.7％ and 30.0％ in Pca tissues with and without lymph node metastasis respectively,and there was a significant difference in the strong positive rate between them (P＜0.05).Conclusions The Oct4 expression is closely related to the differentiation and invasion of tumor.Oct4 might be an important index for the prognosis of prostate cancer.
