CAJ | 학술논문

目的：研究颈椎黄韧带叠压兔黄韧带中转化生长因子β1（transforming growth factor-β1，TGF-β1）的表达变化。方法48只新西兰大白兔，随机分为模型组和对照组，每组24只动物。模型组通过颈椎前路穿刺破坏纤维环及抽吸颈5/6髓核组织建立兔颈椎黄韧带叠压动物模型，术后4、8、12周3个时间点处死动物取材，切取颈5/6后方黄韧带组织，制作石蜡切片并做免疫组织化学检查及图像分析。结果模型组黄韧带纤维排列紊乱，玻璃样变性，TGF-β1表达升高，与对照组相比差异有显著统计学意义（P＜0.05），其中术后4周组变化最为明显，术后8、12周TGF-β1的表达较术后4周降低，差异无统计学意义。结论 TGF-β1在颈椎黄韧带叠压兔黄韧带中的表达增加，加速了黄韧带的退变。
목적：연구경추황인대첩압토황인대중전화생장인자β1（transforming growth factor-β1，TGF-β1）적표체변화。방법48지신서란대백토，수궤분위모형조화대조조，매조24지동물。모형조통과경추전로천자파배섬유배급추흡경5/6수핵조직건립토경추황인대첩압동물모형，술후4、8、12주3개시간점처사동물취재，절취경5/6후방황인대조직，제작석사절편병주면역조직화학검사급도상분석。결과모형조황인대섬유배렬문란，파리양변성，TGF-β1표체승고，여대조조상비차이유현저통계학의의（P＜0.05），기중술후4주조변화최위명현，술후8、12주TGF-β1적표체교술후4주강저，차이무통계학의의。결론 TGF-β1재경추황인대첩압토황인대중적표체증가，가속료황인대적퇴변。
Objective To study TGF-β1 expressed in folding compression of cervical ligamentum flavum animal models .Methods Forty-eight mature New Zealand white rabbits (3000 ± 250g) were divided into two groups ran-domly ,with 24 rats in control group and 24 rats in model group .The folding compression of cervical ligamentum flavum animal models were made by destroyed annulus fibrosus and absorbed nucleus pulposus .Eight rabbits in each group were killed four weeks ,eight weeks and twelve weeks after operation .The ligamentum flavum of C5/6 were collected ,embedded in paraffin and cut into slices .The slices were treated by immunohistochemistry and the image analysis .Results Fibers derangement and hyaline degeneration in ligamentum flavum was found in model group . The expression of TGF-β1 in model group was higher than control group ,and the difference was significant ( P<0 . 05) .After operation for 8 and 12 weeks in the model group the expression of TGF-β1was decreased than those of 4 weeks .But there was no significant difference in statistics .Conclusion Expression of TGF-β1 increased in folding compression of cervical ligamentum flavum animal models and accelerate the degeneration of the ligamentum fla-vum .