CAJ | 학술논문

堆肥化过程中，氨氧化微生物对堆肥原料的氮素转化和氮损失影响重大。为了分析牛粪堆肥高温期微生物的多样性，研究以氨单加氧酶基因（amoA）为标记，分析了牛粪堆肥高温阶段氨氧化古菌（Ammonia-Oxidizing Archaea，AOA）和氨氧化细菌（Ammonia-Oxidizing Bacteria，AOB）菌群多样性。结果表明，在AOB类群中，亚硝化单胞菌属（Nitrosomonas）和亚硝化螺菌属（Nitrosospira）克隆子数量分别占整个克隆文库的59.3%和40.7%，它们是堆肥高温期的优势氨氧化细菌，但是Nitrosomonas的数量比Nitrosospira更占优势。在AOA群落中，soil/sediment菌群占据绝对数量优势，其克隆子数量占AOA文库的94.2%，sea/sediment菌群仅占5.8%。
퇴비화과정중，안양화미생물대퇴비원료적담소전화화담손실영향중대。위료분석우분퇴비고온기미생물적다양성，연구이안단가양매기인（amoA）위표기，분석료우분퇴비고온계단안양화고균（Ammonia-Oxidizing Archaea，AOA）화안양화세균（Ammonia-Oxidizing Bacteria，AOB）균군다양성。결과표명，재AOB류군중，아초화단포균속（Nitrosomonas）화아초화라균속（Nitrosospira）극륭자수량분별점정개극륭문고적59.3%화40.7%，타문시퇴비고온기적우세안양화세균，단시Nitrosomonas적수량비Nitrosospira경점우세。재AOA군락중，soil/sediment균군점거절대수량우세，기극륭자수량점AOA문고적94.2%，sea/sediment균군부점5.8%。
The transmutation and loss of nitrogen materials were effected significantly by ammonia-oxidizing microorganisms during the composting process. In order to detect the diversity of Ammonia-Oxidizing Archaea (AOA)and Ammonia-Oxidizing Bacteria (AOB),the monooxygenase gene (amoA)was used to analyze as the tag at high-temperature phase during the livestock manure composting process. The results showed that Nitrosomonas genus and Nitrosospira genus were the dominant genus of AOB,and the amount of clones accounted for 59.3% and 40.7% among the clone library at the high-temperature period,respectively,and the amount of Nitrosomonas was more dominant than that of the Nitrosospira. For communities of AOA,the amount of AOA resulting from soil were greatly more than that of AOA resulting from sea. The ratio of clones of soil AOA was 94.2%,but the ratio of sea AOA accounted for only 5.8%among all of AOA clones.