浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
15期
1307-1309
,共3页
诸靖宇%王彦彬%宋晨%楼扬锋
諸靖宇%王彥彬%宋晨%樓颺鋒
제정우%왕언빈%송신%루양봉
透明质酸钠%脊髓损伤%神经源膀胱%膀胱黏膜%修复
透明質痠鈉%脊髓損傷%神經源膀胱%膀胱黏膜%脩複
투명질산납%척수손상%신경원방광%방광점막%수복
Sodium hyaluronate%Spinal cord injury%Neurogenic bladder%Bladder mucosa%Repair
目的:探讨透明质酸钠对大鼠脊髓损伤后膀胱黏膜修复的影响。方法将48只大鼠随机分为正常对照组、模型对照组及透明质酸钠灌注组,每组16只。采用脊髓横断法建立大鼠脊髓损伤的神经源性膀胱模型。通过观察各组大鼠膀胱大体情况、HE染色情况、组织学损伤评分以及膀胱组织中IL-6的表达情况评估其炎症的严重程度。结果正常对照组膀胱组织无明显病理改变;模型对照组膀胱组织可见大量中性粒细胞浸润,黏膜严重充血、出血,上皮细胞坏死,黏膜、黏膜下层及肌层均有炎性水肿;透明质酸钠灌注组膀胱组织可见黏膜充血,少量炎性细胞浸润。模型对照组及透明质酸钠灌注组组织学损伤评分及膀胱组织中IL-6阳性表达率均显著高于正常对照组,透明质酸钠灌注组组织学损伤评分及膀胱组织中IL-6阳性表达率明显低于模型对照组,差异均有统计学意义(P<0.05或0.01)。结论透明质酸钠膀胱灌注可以减轻大鼠脊髓损伤后膀胱黏膜急性炎症反应以及促进损伤后的膀胱黏膜修复。
目的:探討透明質痠鈉對大鼠脊髓損傷後膀胱黏膜脩複的影響。方法將48隻大鼠隨機分為正常對照組、模型對照組及透明質痠鈉灌註組,每組16隻。採用脊髓橫斷法建立大鼠脊髓損傷的神經源性膀胱模型。通過觀察各組大鼠膀胱大體情況、HE染色情況、組織學損傷評分以及膀胱組織中IL-6的錶達情況評估其炎癥的嚴重程度。結果正常對照組膀胱組織無明顯病理改變;模型對照組膀胱組織可見大量中性粒細胞浸潤,黏膜嚴重充血、齣血,上皮細胞壞死,黏膜、黏膜下層及肌層均有炎性水腫;透明質痠鈉灌註組膀胱組織可見黏膜充血,少量炎性細胞浸潤。模型對照組及透明質痠鈉灌註組組織學損傷評分及膀胱組織中IL-6暘性錶達率均顯著高于正常對照組,透明質痠鈉灌註組組織學損傷評分及膀胱組織中IL-6暘性錶達率明顯低于模型對照組,差異均有統計學意義(P<0.05或0.01)。結論透明質痠鈉膀胱灌註可以減輕大鼠脊髓損傷後膀胱黏膜急性炎癥反應以及促進損傷後的膀胱黏膜脩複。
목적:탐토투명질산납대대서척수손상후방광점막수복적영향。방법장48지대서수궤분위정상대조조、모형대조조급투명질산납관주조,매조16지。채용척수횡단법건립대서척수손상적신경원성방광모형。통과관찰각조대서방광대체정황、HE염색정황、조직학손상평분이급방광조직중IL-6적표체정황평고기염증적엄중정도。결과정상대조조방광조직무명현병리개변;모형대조조방광조직가견대량중성립세포침윤,점막엄중충혈、출혈,상피세포배사,점막、점막하층급기층균유염성수종;투명질산납관주조방광조직가견점막충혈,소량염성세포침윤。모형대조조급투명질산납관주조조직학손상평분급방광조직중IL-6양성표체솔균현저고우정상대조조,투명질산납관주조조직학손상평분급방광조직중IL-6양성표체솔명현저우모형대조조,차이균유통계학의의(P<0.05혹0.01)。결론투명질산납방광관주가이감경대서척수손상후방광점막급성염증반응이급촉진손상후적방광점막수복。
Objective To investigate the effect of sodium hyaluronate on bladder mucosal repair after spinal cord injury in rats. Methods Forty eight rats were randomly divided into 3 groups with 16 in each. No any treatment for rats in group A (normal control);neurogenic bladder model of spinal cord injury was induced with spinal cord transection in groups B (model group) and C (treatment group), then rats in group C was intravesically infused with sodium hyaluronate. The severity of inflammation in blad-der mucosa was evaluated by HE staining, histological injury score and IL- 6 expression. Results Light microscope showed large number of inflammatory cellinfiltration, significant expansion of capil ary, epithelial cellnecrosis in group B;less inflammato-ry cellinfiltration and capil ary dilatation in group C. Histological injury score in group B was significantly higher than that in group C(P<0.05). The expression of IL- 6 in group A was significantly lower than that in groups B and C(P<0.01);while IL- 6 expression in group C was lower than that in group B(P<0.05). Conclusion Intravesical infusion of sodium hyaluronate can relieve acute in-flammation and enhance the repairing of bladder mucosa in rats after spinal cord injury.