CAJ | 학술논문

【目的】研究Ghrelin在鸭胸腺发育过程中的定位分布及变化规律，以期为进一步探讨Ghrelin对胸腺生理功能的影响提供形态学资料。【方法】选用不同发育阶段的天府肉鸭，胚胎期分7个组，即：14、16、18、20、22、24和26 d胚龄组；胚后期分12个组，即：新生雏、1、3、5、8、11、14、17、20、26、29和32周龄组。运用免疫组织化学技术及形态计量方法研究Ghrelin在鸭胸腺发育过程中的定位分布及变化规律。【结果和结论】Ghrelin免疫阳性细胞弥散分布于20 d胚龄至胚后32周龄的胸腺小叶皮质和髓质；阳性细胞呈圆形、卵圆形、星形或不规则形，主要为胸腺上皮细胞和巨噬细胞。阳性细胞数量在胚胎期无明显差异，在胚后胸腺继续发育期（新生雏～8周龄）和成熟期（11～17周龄）呈波浪状变化，在退化期（20～32周龄）呈下降趋势。说明在天府肉鸭胸腺个体发育中存在Ghrelin免疫阳性细胞，其数量表现出明显增龄变化特性，与胸腺退化密切相关。
【목적】연구Ghrelin재압흉선발육과정중적정위분포급변화규률，이기위진일보탐토Ghrelin대흉선생리공능적영향제공형태학자료。【방법】선용불동발육계단적천부육압，배태기분7개조，즉：14、16、18、20、22、24화26 d배령조；배후기분12개조，즉：신생추、1、3、5、8、11、14、17、20、26、29화32주령조。운용면역조직화학기술급형태계량방법연구Ghrelin재압흉선발육과정중적정위분포급변화규률。【결과화결론】Ghrelin면역양성세포미산분포우20 d배령지배후32주령적흉선소협피질화수질；양성세포정원형、란원형、성형혹불규칙형，주요위흉선상피세포화거서세포。양성세포수량재배태기무명현차이，재배후흉선계속발육기（신생추～8주령）화성숙기（11～17주령）정파랑상변화，재퇴화기（20～32주령）정하강추세。설명재천부육압흉선개체발육중존재Ghrelin면역양성세포，기수량표현출명현증령변화특성，여흉선퇴화밀절상관。
[Objective] The immunohistochemical localization and developmental changes of ghrelin in the duck thymus were studied to provide the morphological effect of ghrelin on the physiological function of thymus.[Method] Tianfu ducks were divided into 19 groups as follows:7 groups in the embryonic peri-od (14, 16, 18, 20, 22, 24 and 26 days at embryonic stage ), 12 groups after hatching ( at neonatal stage, 1, 3, 5, 8, 11, 14, 17, 20, 26, 29 and 32 weeks).The immunohistochemical method associat-ed with morphometric analysis was used .[Result and conclusion] The ghrelin immunoreactivity (-ir) cells with round, ovoid, stellate and irregular aspects were maily thymic epitheilial cells and macropha-ges, and diffusely distributed in the thymic cortex and medulla from embryonic 20 days to 32 weeks after hatching .The number of ghrelin-ir cells in the thymic cortex and medulla was unchanged during the em-bryonic development , presenting a wave-changed pattern during the continuous development ( from neo-natal stage to 8 weeks after hatching ) and matured periods ( from 11 to 17 weeks after hatching ) , which decreased during the involution stage ( from 20 to 32 weeks after hatching ) .These results suggested that ghrelin-ir cells existed in the duck thymus and their numbers exhibited obvious age -related changes , which can be closely related to the involution of the duck thymus .