CAJ | 학술논문

为筛选新的益生菌菌源，从罗非鱼养殖系统分离筛选6株有机物降解菌，研究其对罗非鱼饲料浸出液和饲料原液的降解能力以及菌株对碳源的利用能力。结果表明6株菌对饲料浸出液的有机物均有明显的降解作用，实验72 h后，菌株D51对饲料浸出液有机物的降解率最高，达53.49％，其次为菌株D11，降解率为48.83％。各菌株对饲料原液的有机物均具有明显降解作用，菌株D51、D11和D45的降解效果最好，实验15 d饲料原液的COD分别降低了52.46％、46.03％和46.03％，饲料干重分别减少了58.25％、53.08％和52.08％。各菌株对碳源的利用能力有所差异，菌株D45对31种碳源表现出很强的代谢活性，菌株D51和D11次之，菌株D52和D53对碳源的利用能力相对较低。经分子生物学鉴定，菌株D11属于芽孢杆菌属（Bacillus sp.），菌株D45、D51、D52和D53属于微小杆菌属（Exiguobacterium sp.）。鉴于菌株D11、D45和D51对有机物具有较强的降解能力，可将其作为降解养殖池塘有机物的备选菌株开展后续研究。
위사선신적익생균균원，종라비어양식계통분리사선6주유궤물강해균，연구기대라비어사료침출액화사료원액적강해능력이급균주대탄원적이용능력。결과표명6주균대사료침출액적유궤물균유명현적강해작용，실험72 h후，균주D51대사료침출액유궤물적강해솔최고，체53.49％，기차위균주D11，강해솔위48.83％。각균주대사료원액적유궤물균구유명현강해작용，균주D51、D11화D45적강해효과최호，실험15 d사료원액적COD분별강저료52.46％、46.03％화46.03％，사료간중분별감소료58.25％、53.08％화52.08％。각균주대탄원적이용능력유소차이，균주D45대31충탄원표현출흔강적대사활성，균주D51화D11차지，균주D52화D53대탄원적이용능력상대교저。경분자생물학감정，균주D11속우아포간균속（Bacillus sp.），균주D45、D51、D52화D53속우미소간균속（Exiguobacterium sp.）。감우균주D11、D45화D51대유궤물구유교강적강해능력，가장기작위강해양식지당유궤물적비선균주개전후속연구。
Enhanced degradation of organic matter in aquaculture systems has drawn increasing interests. A study was performed to screen and identify organic-matter-degrading probiotic bacteria from tilapia culture system using 16S rDNA method. Their ability to degrade and utilize organic carbon in leachate and original solution of tilapia feed was also determined using COD and ECO microplate method, respec-tively. Six strains were found to significantly degrade organic matter in the leachates. After 72 h incubation, the degradation rates of organic matter were up to 53.49%for the strain D51, followed by the strain D11(48.83%). The strains D51, D11 and D45 also had high abilities to degrade organic matter in the original feed solution. After 15 d incubation, they decreased COD values in the original feed solution by 52.46%, 46.03%and 46.03%, respectively. The strain D45 showed significantly stronger carbon utilization than the other strains did, with the strains D52 and D53 being the lowest. 16S rDNA identification showed that D11 was Bacillus sp. and the strain D45, D51, D52 and D53 were Exiguobacterium sp. These results suggest that the strains D11, D45 and D51 have great organic degradation potential and warrant more indepth studies.