药品评价
藥品評價
약품평개
DRUG REEVALUATION
2013年
24期
24-27
,共4页
舒胸片%人参皂苷Rg1%人参皂苷Rb1%三七皂苷R1%HPLC法
舒胸片%人參皂苷Rg1%人參皂苷Rb1%三七皂苷R1%HPLC法
서흉편%인삼조감Rg1%인삼조감Rb1%삼칠조감R1%HPLC법
Shuxiong Tablet%Ginsenoside Rg1%Ginsenoside Rb1%Notoginsenoside R1%HPLC
目的：建立舒胸片中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1含量的HPLC测定方法。方法：采用DiamosilC18柱(4.6×250mm，5μm)，流动相乙腈-水，梯度洗脱，柱温30℃，流速1mL/min，203nm波长下检测。结果：人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1分别在0.442～11.050μg(r=0.9999)、0.344～8.600μg(r=0.9999)、0.208～5.200μg(r=0.9995)范围内线性关系良好，平均回收率分别为98.93%(RSD为1.7%)、98.88%(RSD为1.4%)、98.98%(RSD为1.4%)。结论：以HPLC法检测舒胸片中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1，方法简便、准确、灵敏度高、重复性好。
目的：建立舒胸片中人參皂苷Rg1、人參皂苷Rb1和三七皂苷R1含量的HPLC測定方法。方法：採用DiamosilC18柱(4.6×250mm，5μm)，流動相乙腈-水，梯度洗脫，柱溫30℃，流速1mL/min，203nm波長下檢測。結果：人參皂苷Rg1、人參皂苷Rb1、三七皂苷R1分彆在0.442～11.050μg(r=0.9999)、0.344～8.600μg(r=0.9999)、0.208～5.200μg(r=0.9995)範圍內線性關繫良好，平均迴收率分彆為98.93%(RSD為1.7%)、98.88%(RSD為1.4%)、98.98%(RSD為1.4%)。結論：以HPLC法檢測舒胸片中人參皂苷Rg1、人參皂苷Rb1和三七皂苷R1，方法簡便、準確、靈敏度高、重複性好。
목적：건립서흉편중인삼조감Rg1、인삼조감Rb1화삼칠조감R1함량적HPLC측정방법。방법：채용DiamosilC18주(4.6×250mm，5μm)，류동상을정-수，제도세탈，주온30℃，류속1mL/min，203nm파장하검측。결과：인삼조감Rg1、인삼조감Rb1、삼칠조감R1분별재0.442～11.050μg(r=0.9999)、0.344～8.600μg(r=0.9999)、0.208～5.200μg(r=0.9995)범위내선성관계량호，평균회수솔분별위98.93%(RSD위1.7%)、98.88%(RSD위1.4%)、98.98%(RSD위1.4%)。결론：이HPLC법검측서흉편중인삼조감Rg1、인삼조감Rb1화삼칠조감R1，방법간편、준학、령민도고、중복성호。
Objective: To develop a HPLC method for the qualification and quantification of ginsenoside Rg1, Rb1 and notoginsenoside R1 in Shuxiong Tablet. Methods: The column was Diamosil C18 (4.6×250mm, 5μm). The gradient elution mixture consisted of acetonitrile and water. The flow rate was 1mL/min, and the column temperature and detection wavelength were 30℃ and 203 nm, respectively. Results: Linear ranges were 0.442~11.050 μg (r=0.9999) for ginsenoside Rg1,0.344~8.600 μg (r=0.9999) for ginsenoside Rb1, 0.208~5.200μg (r=0.9995) for notoginsenoside R1. The average recovery was 98.93%with RSD 1.7%for ginsenoside Rg1, 98.88% with RSD 1.4% for ginsenoside Rb1,98.98% with RSD 1.4% for notoginsenoside R1. Conclusion:The established HPLC method is convenient, accurate, sensitive and repeatable.
