CAJ | 학술논문

目的：观察大鼠肢体缺血再灌注（LIR）后胰腺的变化及牛磺酸（Tau）后处理的保护作用。方法将24只SD雄性大鼠随机分为对照（Control）组、LIR组和Tau组，每组8只。测定各组血浆黄嘌呤氧化酶（XOD）、丙二醛（MDA）、活性氧（ROS）、超氧化物歧化酶（SOD）、胰淀粉酶（AMS）、胰脂肪酶（LPS）、胰蛋白酶（Trypsin）含量的变化，光镜下观察胰腺组织形态学变化，用免疫组化观察胰腺组织中C/EBP同源蛋白（CHOP）的表达，TUNEL法观察胰腺组织细胞凋亡情况。结果 LIR组MDA、XOD、ROS、AMS、LPS及Trypsin均高于Control组和Tau组，SOD低于Control组和Tau组（均P＜0.05）。HE染色结果显示LIR组腺泡结构破坏，间质血管扩张充血,血管周围有炎细胞浸润，胰岛形态不规则。Tau组较LIR组腺泡分界略清楚，血管扩张程度减轻，周围炎细胞减少，核浆分界和胰岛结构均有改善。免疫组化染色结果显示Tau组胰腺组织CHOP表达较LIR组下调。TUNEL染色结果显示Tau组胰腺组织细胞凋亡数量较LIR组减少。结论 Tau后处理可减轻大鼠LIR后胰腺损伤，其机制可能与抑制氧化应激反应、下调CHOP表达，从而减轻内质网应激诱导的细胞凋亡有关。
목적：관찰대서지체결혈재관주（LIR）후이선적변화급우광산（Tau）후처리적보호작용。방법장24지SD웅성대서수궤분위대조（Control）조、LIR조화Tau조，매조8지。측정각조혈장황표령양화매（XOD）、병이철（MDA）、활성양（ROS）、초양화물기화매（SOD）、이정분매（AMS）、이지방매（LPS）、이단백매（Trypsin）함량적변화，광경하관찰이선조직형태학변화，용면역조화관찰이선조직중C/EBP동원단백（CHOP）적표체，TUNEL법관찰이선조직세포조망정황。결과 LIR조MDA、XOD、ROS、AMS、LPS급Trypsin균고우Control조화Tau조，SOD저우Control조화Tau조（균P＜0.05）。HE염색결과현시LIR조선포결구파배，간질혈관확장충혈,혈관주위유염세포침윤，이도형태불규칙。Tau조교LIR조선포분계략청초，혈관확장정도감경，주위염세포감소，핵장분계화이도결구균유개선。면역조화염색결과현시Tau조이선조직CHOP표체교LIR조하조。TUNEL염색결과현시Tau조이선조직세포조망수량교LIR조감소。결론 Tau후처리가감경대서LIR후이선손상，기궤제가능여억제양화응격반응、하조CHOP표체，종이감경내질망응격유도적세포조망유관。
Objective To observe changes of the pancreas after limb ischemia reperfusion, and the protective effect of taurine (Tau) postconditioning. Methods Twenty-four male SD rats were randomly divided into control group (Control), limb ischemia reperfusion (LIR) group, and taurine postconditioning (Tau) group (n=8 for each group). Plasma contents of xanthine oxidase (XOD), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), pancreatic amylase (AMS), pancreatic lipase (LPS) and trypsin were detected in three groups. Morphological changes of the pancreas were observed using optical microscopy. The expressions of anti-C/enhancer-binding protein homologous protein (CHOP) in pancreas were analyzed by immunohistochemistry assay. TUNEL staining was performed to estimate the apoptosis of pancre-atic cells. Results Compared with the control group and Tau group, plasma contents of MDA, XOD, ROS, AMS, LPS, and trypsin were significantly increased in LIR group, but the level of SOD was significantly lower in LIR group ( P<0.05). HE staining showed that acinar structure was disrupted , pancreatic lobule gap widened, stromal vascular dilatation and conges-tion were observed in LIR group. The perivascular infiltration of inflammatory cells appeared, islet cells were damaged with irregular islet. The immunohistochemical analysis showed the increased expression of CHOP in pancreas in LIR group than that of Tau group. And the pancreatic apoptosis was enhanced in LIR group detected by TUNEL staining. Conclusion Tau-rine postconditioning can ameliorate pancreatic injury following limb ischemia reperfusion, which may be related to the inhi-bition of oxidative stress, down-regulation of CHOP expression, thereby reducing endoplasmic reticulum stress-induced apoptosis.