天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2013年
12期
1184-1187
,共4页
薛雅馨%仇小强%余红平%谭盛葵
薛雅馨%仇小彊%餘紅平%譚盛葵
설아형%구소강%여홍평%담성규
柯萨奇病毒感染%叶酸%心脏缺损,先天性%GATA4转录因子%逆转录聚合酶链反应%印迹法,蛋白质%大鼠, Sprague-Dawley%NKx2.5
柯薩奇病毒感染%葉痠%心髒缺損,先天性%GATA4轉錄因子%逆轉錄聚閤酶鏈反應%印跡法,蛋白質%大鼠, Sprague-Dawley%NKx2.5
가살기병독감염%협산%심장결손,선천성%GATA4전록인자%역전록취합매련반응%인적법,단백질%대서, Sprague-Dawley%NKx2.5
coxsackievirus infections%folic acid%heart defects,congenital%GATA4 transcription factor%reverse tran-scriptase polymerase chain reaction%blotting,Western%rats,Sprague-Dawley%NKx2.5
目的：探讨叶酸对孕期感染柯萨奇B3病毒(CVB3)的新生鼠心脏发育因子GATA-4、NKx2.5的基因与蛋白表达的影响。方法 SD雌鼠分为对照组、叶酸组、CVB3组、CVB3+叶酸组。叶酸组及CVB3+叶酸组孕前给予叶酸灌胃2周；CVB3组及CVB3+叶酸组孕第7天腹腔注射CVB3，连续5 d，自然分娩后取新生鼠心脏组织，病理切片HE染色观察新生鼠心脏组织形态学变化；逆转录聚合酶链反应（RT-PCR）及Western blot测定新生鼠心脏组织中转录因子GATA-4、NKx2.5基因及蛋白表达水平。结果 CVB3组新生鼠心肌损伤明显，心肌细胞排列紊乱，心肌纤维断裂，CVB3+叶酸组状态改善。CVB3组新生鼠心脏组织GATA-4、NKx2.5基因及蛋白表达低于对照组（P＜0.05）， CVB3+叶酸组GATA-4、NKx2.5基因及蛋白表达高于CVB3组（P＜0.05）。结论孕鼠早期感染CVB3抑制新生鼠心脏发育因子正常表达，补充叶酸对新生鼠心脏发育具有明显保护作用。
目的：探討葉痠對孕期感染柯薩奇B3病毒(CVB3)的新生鼠心髒髮育因子GATA-4、NKx2.5的基因與蛋白錶達的影響。方法 SD雌鼠分為對照組、葉痠組、CVB3組、CVB3+葉痠組。葉痠組及CVB3+葉痠組孕前給予葉痠灌胃2週；CVB3組及CVB3+葉痠組孕第7天腹腔註射CVB3，連續5 d，自然分娩後取新生鼠心髒組織，病理切片HE染色觀察新生鼠心髒組織形態學變化；逆轉錄聚閤酶鏈反應（RT-PCR）及Western blot測定新生鼠心髒組織中轉錄因子GATA-4、NKx2.5基因及蛋白錶達水平。結果 CVB3組新生鼠心肌損傷明顯，心肌細胞排列紊亂，心肌纖維斷裂，CVB3+葉痠組狀態改善。CVB3組新生鼠心髒組織GATA-4、NKx2.5基因及蛋白錶達低于對照組（P＜0.05）， CVB3+葉痠組GATA-4、NKx2.5基因及蛋白錶達高于CVB3組（P＜0.05）。結論孕鼠早期感染CVB3抑製新生鼠心髒髮育因子正常錶達，補充葉痠對新生鼠心髒髮育具有明顯保護作用。
목적：탐토협산대잉기감염가살기B3병독(CVB3)적신생서심장발육인자GATA-4、NKx2.5적기인여단백표체적영향。방법 SD자서분위대조조、협산조、CVB3조、CVB3+협산조。협산조급CVB3+협산조잉전급여협산관위2주；CVB3조급CVB3+협산조잉제7천복강주사CVB3，련속5 d，자연분면후취신생서심장조직，병리절편HE염색관찰신생서심장조직형태학변화；역전록취합매련반응（RT-PCR）급Western blot측정신생서심장조직중전록인자GATA-4、NKx2.5기인급단백표체수평。결과 CVB3조신생서심기손상명현，심기세포배렬문란，심기섬유단렬，CVB3+협산조상태개선。CVB3조신생서심장조직GATA-4、NKx2.5기인급단백표체저우대조조（P＜0.05）， CVB3+협산조GATA-4、NKx2.5기인급단백표체고우CVB3조（P＜0.05）。결론잉서조기감염CVB3억제신생서심장발육인자정상표체，보충협산대신생서심장발육구유명현보호작용。
Objective To investigate the effects of oral folic acid on cardiac development related gene expression of offspring in an experimental model of coxsackievirus B3(CVB3) infection of pregnant rats. Methods SD female rats were randomized into control group, folic acid group, CVB3 group and CVB3+folic acid group. The female rats were given folic acid by gavage for 2 weeks before pregnancy in folic acid group and CVB3+folic acid group. After conception for 7 days, rat model was established by intraperitoneal injection of CVB3 for 5 days in CVB3 group and CVB3+folic acid group. After nat-ural childbirth neonatal heart was taken and stored in liquid nitrogen. The morphological changes of neonatal rat myocardial tissues were observed by HE staining. The expressions of GATA-4 and NKx2.5 mRNA were detected by RT-PCR and West-ern blot assay. Results There was significant myocardial injury, such as myocardial fiber disarray and myocardial fiber breakage, in neonatal rats in CVB3 group. These damages were improved in CVB3+folic acid group. The expression levels of GATA-4 and NKx2.5 genes in myocardial tissues were significantly lower in CVB3 group than those of control group (P<0.05). The expression levels of GATA-4 and NKx2.5 proteins in myocardial tissues were significantly higher in CVB3+folic acid group than those of CVB3 group (P<0.05). Conclusion CVB3 infection in the early pregnancy inhibited the expres-sion of neonatal rat cardiac development factor. Folic acid supplementation has obvious protective effects on the neonatal rat cardiac development.
