中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
5期
782-785
,共4页
王玉娟%陶利%崔苏镇%梁竹%周金辉
王玉娟%陶利%崔囌鎮%樑竹%週金輝
왕옥연%도리%최소진%량죽%주금휘
伤痛酊%氯化两面针碱%乙氧基白屈菜红碱%岩白菜素%百两金皂苷A%HPLC-DAD-ELSD法
傷痛酊%氯化兩麵針堿%乙氧基白屈菜紅堿%巖白菜素%百兩金皂苷A%HPLC-DAD-ELSD法
상통정%록화량면침감%을양기백굴채홍감%암백채소%백량금조감A%HPLC-DAD-ELSD법
Shangtong tinctures%Nitidine chloride%5-Ethoxychelerythrine%Bergeninum%Ardisiacrispin A%HPLC-DAD-ELSD
目的：采用HPLC-DAD-ELSD法测定伤痛酊( STD)中氯化两面针碱、乙氧基白屈菜红碱、岩白菜素和百两金皂苷A的含量。方法：采用Hypersil C18色谱柱(200 mm ×4.6 mm,5μm)；流速：0.8 ml·min-1；氯化两面针碱和乙氧基白屈菜红碱：流动相A为乙腈,流动相B为0.1%甲酸-三乙胺(pH 4.5),检测波长为273 nm。岩白菜素和百两金皂苷A：甲醇-水(25∶75)为流动相；漂移管温度：95℃,载气( N2)流速：2.5 SLPM·min-1。结果：氯化两面针碱与乙氧基白屈菜红碱进样量分别在0.021~0.426μg(r=0.9995)、0.075~1.494μg(r=0.9998)与峰面积呈良好的线性关系,平均加样回收率分别为99.22%、98.61%,RSD分别为0.64%、0.46%(n=9)；岩白菜素和百两金皂苷A分别在0.215~4.304(r=0.9993)、0.286~5.728μg (r=0.9997)进样量的自然对数值与峰面积的自然对数值良好的线性关系,平均加样回收率分别为99.15%、99.25%,RSD分别为0.77%、0.56%(n=9)。结论：该测定方法结果准确、灵敏度高、重复性好,可用于伤痛酊中氯化两面针碱、乙氧基白屈菜红碱、岩白菜素和百两金皂苷A的含量测定。
目的：採用HPLC-DAD-ELSD法測定傷痛酊( STD)中氯化兩麵針堿、乙氧基白屈菜紅堿、巖白菜素和百兩金皂苷A的含量。方法：採用Hypersil C18色譜柱(200 mm ×4.6 mm,5μm)；流速：0.8 ml·min-1；氯化兩麵針堿和乙氧基白屈菜紅堿：流動相A為乙腈,流動相B為0.1%甲痠-三乙胺(pH 4.5),檢測波長為273 nm。巖白菜素和百兩金皂苷A：甲醇-水(25∶75)為流動相；漂移管溫度：95℃,載氣( N2)流速：2.5 SLPM·min-1。結果：氯化兩麵針堿與乙氧基白屈菜紅堿進樣量分彆在0.021~0.426μg(r=0.9995)、0.075~1.494μg(r=0.9998)與峰麵積呈良好的線性關繫,平均加樣迴收率分彆為99.22%、98.61%,RSD分彆為0.64%、0.46%(n=9)；巖白菜素和百兩金皂苷A分彆在0.215~4.304(r=0.9993)、0.286~5.728μg (r=0.9997)進樣量的自然對數值與峰麵積的自然對數值良好的線性關繫,平均加樣迴收率分彆為99.15%、99.25%,RSD分彆為0.77%、0.56%(n=9)。結論：該測定方法結果準確、靈敏度高、重複性好,可用于傷痛酊中氯化兩麵針堿、乙氧基白屈菜紅堿、巖白菜素和百兩金皂苷A的含量測定。
목적：채용HPLC-DAD-ELSD법측정상통정( STD)중록화량면침감、을양기백굴채홍감、암백채소화백량금조감A적함량。방법：채용Hypersil C18색보주(200 mm ×4.6 mm,5μm)；류속：0.8 ml·min-1；록화량면침감화을양기백굴채홍감：류동상A위을정,류동상B위0.1%갑산-삼을알(pH 4.5),검측파장위273 nm。암백채소화백량금조감A：갑순-수(25∶75)위류동상；표이관온도：95℃,재기( N2)류속：2.5 SLPM·min-1。결과：록화량면침감여을양기백굴채홍감진양량분별재0.021~0.426μg(r=0.9995)、0.075~1.494μg(r=0.9998)여봉면적정량호적선성관계,평균가양회수솔분별위99.22%、98.61%,RSD분별위0.64%、0.46%(n=9)；암백채소화백량금조감A분별재0.215~4.304(r=0.9993)、0.286~5.728μg (r=0.9997)진양량적자연대수치여봉면적적자연대수치량호적선성관계,평균가양회수솔분별위99.15%、99.25%,RSD분별위0.77%、0.56%(n=9)。결론：해측정방법결과준학、령민도고、중복성호,가용우상통정중록화량면침감、을양기백굴채홍감、암백채소화백량금조감A적함량측정。
Objective:To develop an HPLC-DAD-ELSD method for the determination of nitidine chloride, 5-ethoxychelerythrine, bergeninum and ardisiacrispin A in Shangtong tinctures ( STD) . Methods: A Hypersil C18 column was used as the chromatographic column, the flow rate was 0. 8 ml·min-1 . For nitidine chloride and 5-ethoxychelerythrine, the mobile phase A consisted of acetoni-trile,the mobile phase B consisted of 0. 1% formic acid-triethylamine (pH 4. 5),and the DAD detection wavelength was at 273 nm. For bergeninum and ardisiacrispin A, the mobile phase consisted of methanol-water(25∶75), the temperature of drift tube was set at 95℃, and the gas flow (N2) was set at 2. 5 SLPM·min-1. Results:There was a good linear relationship between the concentration and peak area for nitidine chloride and 5-ethoxychelerythrine within the range of 0. 021-0. 426 μg (r=0. 999 5) and 0. 075-1. 494 μg (r=0. 999 8), respectively. The average recovery was 99. 22%(RSD=0. 64%) and 98. 61%(RSD=0. 46%), respectively. There was a good linear relationship between the concentration and peak area for bergeninum and ardisiacrispin A within the range of 0. 215-4. 304 μg(r=0. 999 3) and 0. 286-5. 728 μg(r=0. 999 7), respectively. The average recovery was 99. 15%(RSD=0. 77%) and 99. 25%(RSD=0. 56%) accordingly. Conclusion:The method is accurate, sensitive and reproducible, and can be used in the de-termination of nitidine chloride, 5-ethoxychelerythrine, bergeninum and ardisiacrispin A in STD.
