中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
2期
81-85
,共5页
魏新素%张平安%童永清%韦传东%祝成亮
魏新素%張平安%童永清%韋傳東%祝成亮
위신소%장평안%동영청%위전동%축성량
肝炎,丙型,慢性%Toll样受体%多态性,单核苷酸
肝炎,丙型,慢性%Toll樣受體%多態性,單覈苷痠
간염,병형,만성%Toll양수체%다태성,단핵감산
Chronic hepatitis C%Toll-like receptors%Polymorphism,single nucleotide
目的 分析慢性丙型肝炎病毒(HCV)感染与Toll样受体7(TLR7)和Toll样受体9(TLR9)单核苷酸多态性(SNP)的相关性.方法 选择2011年1月至2012年5月武汉大学人民医院150例慢性丙型肝炎(CHC)患者及同期体检的168名健康对照者.采用Sanger测序法检测TLR7IVS2-151(rs179009)基因型,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测TLR9T-1486C(rs187084)基因型.采用SPSS 15.0软件进行统计学分析,对基因型进行Hardy-Weinberg平衡吻合度检验.结果 TLR7 IVS2-151G频率在男性CHC患者中高于对照组男性(41.4％∶21.6％,x2=7.250,P=0.007,OR=0.389,95％ CI:0.194 ～0.781)；TLR7 IVS2-151A的频率在女性CHC患者中显著高于对照组女性(76.9％∶63.1％,x2=7.202,P=0.007, OR=1.942,95％ CI:1.192 ～3.164).TLR9 T-1486C (rs187084)位点不同基因型和等位基因在CHC组和健康对照组间的分布频率差异均无统计学意义(P＞0.05).结论 TLR7 IVS2-151 (rs179009)位点与HCV感染存在相关性,其可能参与了CHC的发病.
目的 分析慢性丙型肝炎病毒(HCV)感染與Toll樣受體7(TLR7)和Toll樣受體9(TLR9)單覈苷痠多態性(SNP)的相關性.方法 選擇2011年1月至2012年5月武漢大學人民醫院150例慢性丙型肝炎(CHC)患者及同期體檢的168名健康對照者.採用Sanger測序法檢測TLR7IVS2-151(rs179009)基因型,應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法檢測TLR9T-1486C(rs187084)基因型.採用SPSS 15.0軟件進行統計學分析,對基因型進行Hardy-Weinberg平衡吻閤度檢驗.結果 TLR7 IVS2-151G頻率在男性CHC患者中高于對照組男性(41.4％∶21.6％,x2=7.250,P=0.007,OR=0.389,95％ CI:0.194 ～0.781)；TLR7 IVS2-151A的頻率在女性CHC患者中顯著高于對照組女性(76.9％∶63.1％,x2=7.202,P=0.007, OR=1.942,95％ CI:1.192 ～3.164).TLR9 T-1486C (rs187084)位點不同基因型和等位基因在CHC組和健康對照組間的分佈頻率差異均無統計學意義(P＞0.05).結論 TLR7 IVS2-151 (rs179009)位點與HCV感染存在相關性,其可能參與瞭CHC的髮病.
목적 분석만성병형간염병독(HCV)감염여Toll양수체7(TLR7)화Toll양수체9(TLR9)단핵감산다태성(SNP)적상관성.방법 선택2011년1월지2012년5월무한대학인민의원150례만성병형간염(CHC)환자급동기체검적168명건강대조자.채용Sanger측서법검측TLR7IVS2-151(rs179009)기인형,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측TLR9T-1486C(rs187084)기인형.채용SPSS 15.0연건진행통계학분석,대기인형진행Hardy-Weinberg평형문합도검험.결과 TLR7 IVS2-151G빈솔재남성CHC환자중고우대조조남성(41.4％∶21.6％,x2=7.250,P=0.007,OR=0.389,95％ CI:0.194 ～0.781)；TLR7 IVS2-151A적빈솔재녀성CHC환자중현저고우대조조녀성(76.9％∶63.1％,x2=7.202,P=0.007, OR=1.942,95％ CI:1.192 ～3.164).TLR9 T-1486C (rs187084)위점불동기인형화등위기인재CHC조화건강대조조간적분포빈솔차이균무통계학의의(P＞0.05).결론 TLR7 IVS2-151 (rs179009)위점여HCV감염존재상관성,기가능삼여료CHC적발병.
Objective To analyze the association of the single nucleotide polymorphism (SNP) of Toll-like receptor 7 (TLR7) and Toll-like receptor 9 (TLR9) with chronic hepatitis C virus (HCV)infection.Methods A total of 150 patients with chronic hepatitis C (CHC) admitted to Renmin Hospital of Wuhan University from January 2011 to May 2012 and 168 healthy controls were enrolled in the study.The genotypes of TLR7 IVS2-151 (rs179009) were detected by Sanger sequencing,and the genotypes of TLR9 T-1486C (rs187084) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).SPSS 15.0 was used for statistical analysis,and goodness-of-fit test for HardyWeinberg equilibrium was also performed.Results The frequency of TLR7 IVS2-151G was higher in malepatients with CHC than that in male controls (41.4％ vs.21.6％,x2 =7.250,P =0.007,OR =0.389,95％ CI:0.194-0.781) ; however the female CHC patients had a higher frequency of TLR7 IVS2-151A than the female controls (76.9％ vs.63.1％,x2 =7.202,P =0.007,OR =1.942,95％ CI:1.192-3.164).No significant difference in the distribution of TLR9 T-1486C (rs187084) gene SNP was observed betweenCHC and control groups (P ＞0.05).Conclusion TLR7 IVS2-151 (rs179009) is correlated with HCV infection,which may be involved in the pathogenesis of CHC.
