目的:探讨长波紫外线(ultraviolet A,UVA)和中波紫外线(ultraviolet B,UVB)对去卵巢骨质疏松(osteoporosis)模型大鼠的血清1,25-二羟基维生素D3[1,25-dihydroxy-vitamin D3,1,25(OH)2D3]和骨代谢的影响,并比较UVA和UVB的治疗效果.方法:将40只健康雌性6月龄SD大鼠随机分为假手术对照组(sham)、模型组(OVX)、UVA照射组(OVX+UVA)、UVB照射组(OVX+ UVB).除假手术对照组外,其余各组经双侧卵巢切除术建立骨质疏松大鼠模型.模型建立后,对UVA照射组和UVB照射组分别使用波长340 nm和313 nm的紫外线进行照射治疗,15周后测定大鼠骨密度及血清1,25(OH)2D3、骨钙素(bone gamma-carboxyglutamic acid protein,BGP)、钙(calcium,Ca)和磷(phosphorus,P)的含量.结果:去卵巢术后6个月,与假手术对照组相比,模型组、UVA照射组和UVB照射组大鼠体重[(486.5±55.7)g,(488.3 ±32.1)g,(494.1 ±49.8)g,vs.(408.6±36.1)g,P＜0.01]显著升高,股骨近心端骨密度[(0.318±0.025)g/cm2,(0.316±0.031) g/cm2,(0.322±0.036) g/cm2,vs.(0.386±0.027) g/cm2,P＜0.01]、中心骨密度[(0.321±0.038) g/cm2,(0.319±0.051) g/cm2,(0.320±0.053) g/cm2,vs.(0.347±0.044) g/cm2,P＜0.05)]和远心端骨密度[(0.320±0.028)g/cm2,(0.318 ±0.030) g/cm2,(0.322±0.036) g/cm2,vs.(0.361±0.046)g/cm2,P＜0.01]显著降低,差异有统计学意义.紫外线照射15周后,与假手术对照组相比,模型组大鼠股骨近心端骨密度[(0.162 ±0.125) g/cm2vs.(0.293±0.076) g/cm2,P＜0.01]、中心骨密度[(0.205±0.102)g/cm2vs.(0.306±0.031) g/cm2,P＜0.01]和远心端骨密度[(0.153±0.119) g/cm2vs.(0.274±0.017) g/cm2,P ＜0.01]显著降低,血清1,25 (OH)2 D3[(19.80±1.67)ng/L vs.(28.35±4.32) ng/L,P＜0.01]、骨钙素[(11.00±0.01) ng/L vs.(16.64±0.01) ng/L,P＜0.01]和钙[(2.14±0.10) mmol/L vs.(2.68±0.16)mmol/L,P＜0.01]含量显著降低,差异有统计学意义.与模型组相比,UVA照射组和UVB照射组股骨近心端骨密度[(0.248±0.092) g/cm2,(0.218±0.123) g/cm2,vs.(0.162±0.125) g/cm2,P＜0.01]、中心骨密度[(0.272±0.010) g/cm2,(0.275±0.036) g/cm2,vs.(0.205±0.102) g/cm2,P＜0.01]、远心端骨密度[(0.251±0.009) g/cm2,(0.242±0.063) g/cm2,vs.(0.153±0.119) g/cm2,P＜0.01]显著升高,血清1,25(OH)2D3[(29.47±4.54) ng/L,(27.56±6.33)ng/L,vs.(19.80±1.67)ng/L,P＜0.01]、骨钙素[(15.70±0.01) ng/L,(15.62±0.02) ng/L,vs.(11.00±0.01) ng/L,P ＜0.01]、钙[(2.48±0.22) mmol/L,(2.58±0.13) mmol/L,vs.(2.14±0.10) mmol/L,P＜0.01]含量显著升高,差异有统计学意义.与UVB照射组相比,UVA照射组大鼠血清1,25(OH)2D3、骨钙素、钙和磷含量及骨密度的差异无统计学意义.结论:UVA和UVB均能提高去卵巢骨质疏松模型大鼠血清1,25(OH)2D3含量,促进骨形成,升高骨密度,缓解骨质疏松造成的骨质丢失.
目的:探討長波紫外線(ultraviolet A,UVA)和中波紫外線(ultraviolet B,UVB)對去卵巢骨質疏鬆(osteoporosis)模型大鼠的血清1,25-二羥基維生素D3[1,25-dihydroxy-vitamin D3,1,25(OH)2D3]和骨代謝的影響,併比較UVA和UVB的治療效果.方法:將40隻健康雌性6月齡SD大鼠隨機分為假手術對照組(sham)、模型組(OVX)、UVA照射組(OVX+UVA)、UVB照射組(OVX+ UVB).除假手術對照組外,其餘各組經雙側卵巢切除術建立骨質疏鬆大鼠模型.模型建立後,對UVA照射組和UVB照射組分彆使用波長340 nm和313 nm的紫外線進行照射治療,15週後測定大鼠骨密度及血清1,25(OH)2D3、骨鈣素(bone gamma-carboxyglutamic acid protein,BGP)、鈣(calcium,Ca)和燐(phosphorus,P)的含量.結果:去卵巢術後6箇月,與假手術對照組相比,模型組、UVA照射組和UVB照射組大鼠體重[(486.5±55.7)g,(488.3 ±32.1)g,(494.1 ±49.8)g,vs.(408.6±36.1)g,P＜0.01]顯著升高,股骨近心耑骨密度[(0.318±0.025)g/cm2,(0.316±0.031) g/cm2,(0.322±0.036) g/cm2,vs.(0.386±0.027) g/cm2,P＜0.01]、中心骨密度[(0.321±0.038) g/cm2,(0.319±0.051) g/cm2,(0.320±0.053) g/cm2,vs.(0.347±0.044) g/cm2,P＜0.05)]和遠心耑骨密度[(0.320±0.028)g/cm2,(0.318 ±0.030) g/cm2,(0.322±0.036) g/cm2,vs.(0.361±0.046)g/cm2,P＜0.01]顯著降低,差異有統計學意義.紫外線照射15週後,與假手術對照組相比,模型組大鼠股骨近心耑骨密度[(0.162 ±0.125) g/cm2vs.(0.293±0.076) g/cm2,P＜0.01]、中心骨密度[(0.205±0.102)g/cm2vs.(0.306±0.031) g/cm2,P＜0.01]和遠心耑骨密度[(0.153±0.119) g/cm2vs.(0.274±0.017) g/cm2,P ＜0.01]顯著降低,血清1,25 (OH)2 D3[(19.80±1.67)ng/L vs.(28.35±4.32) ng/L,P＜0.01]、骨鈣素[(11.00±0.01) ng/L vs.(16.64±0.01) ng/L,P＜0.01]和鈣[(2.14±0.10) mmol/L vs.(2.68±0.16)mmol/L,P＜0.01]含量顯著降低,差異有統計學意義.與模型組相比,UVA照射組和UVB照射組股骨近心耑骨密度[(0.248±0.092) g/cm2,(0.218±0.123) g/cm2,vs.(0.162±0.125) g/cm2,P＜0.01]、中心骨密度[(0.272±0.010) g/cm2,(0.275±0.036) g/cm2,vs.(0.205±0.102) g/cm2,P＜0.01]、遠心耑骨密度[(0.251±0.009) g/cm2,(0.242±0.063) g/cm2,vs.(0.153±0.119) g/cm2,P＜0.01]顯著升高,血清1,25(OH)2D3[(29.47±4.54) ng/L,(27.56±6.33)ng/L,vs.(19.80±1.67)ng/L,P＜0.01]、骨鈣素[(15.70±0.01) ng/L,(15.62±0.02) ng/L,vs.(11.00±0.01) ng/L,P ＜0.01]、鈣[(2.48±0.22) mmol/L,(2.58±0.13) mmol/L,vs.(2.14±0.10) mmol/L,P＜0.01]含量顯著升高,差異有統計學意義.與UVB照射組相比,UVA照射組大鼠血清1,25(OH)2D3、骨鈣素、鈣和燐含量及骨密度的差異無統計學意義.結論:UVA和UVB均能提高去卵巢骨質疏鬆模型大鼠血清1,25(OH)2D3含量,促進骨形成,升高骨密度,緩解骨質疏鬆造成的骨質丟失.
목적:탐토장파자외선(ultraviolet A,UVA)화중파자외선(ultraviolet B,UVB)대거란소골질소송(osteoporosis)모형대서적혈청1,25-이간기유생소D3[1,25-dihydroxy-vitamin D3,1,25(OH)2D3]화골대사적영향,병비교UVA화UVB적치료효과.방법:장40지건강자성6월령SD대서수궤분위가수술대조조(sham)、모형조(OVX)、UVA조사조(OVX+UVA)、UVB조사조(OVX+ UVB).제가수술대조조외,기여각조경쌍측란소절제술건립골질소송대서모형.모형건립후,대UVA조사조화UVB조사조분별사용파장340 nm화313 nm적자외선진행조사치료,15주후측정대서골밀도급혈청1,25(OH)2D3、골개소(bone gamma-carboxyglutamic acid protein,BGP)、개(calcium,Ca)화린(phosphorus,P)적함량.결과:거란소술후6개월,여가수술대조조상비,모형조、UVA조사조화UVB조사조대서체중[(486.5±55.7)g,(488.3 ±32.1)g,(494.1 ±49.8)g,vs.(408.6±36.1)g,P＜0.01]현저승고,고골근심단골밀도[(0.318±0.025)g/cm2,(0.316±0.031) g/cm2,(0.322±0.036) g/cm2,vs.(0.386±0.027) g/cm2,P＜0.01]、중심골밀도[(0.321±0.038) g/cm2,(0.319±0.051) g/cm2,(0.320±0.053) g/cm2,vs.(0.347±0.044) g/cm2,P＜0.05)]화원심단골밀도[(0.320±0.028)g/cm2,(0.318 ±0.030) g/cm2,(0.322±0.036) g/cm2,vs.(0.361±0.046)g/cm2,P＜0.01]현저강저,차이유통계학의의.자외선조사15주후,여가수술대조조상비,모형조대서고골근심단골밀도[(0.162 ±0.125) g/cm2vs.(0.293±0.076) g/cm2,P＜0.01]、중심골밀도[(0.205±0.102)g/cm2vs.(0.306±0.031) g/cm2,P＜0.01]화원심단골밀도[(0.153±0.119) g/cm2vs.(0.274±0.017) g/cm2,P ＜0.01]현저강저,혈청1,25 (OH)2 D3[(19.80±1.67)ng/L vs.(28.35±4.32) ng/L,P＜0.01]、골개소[(11.00±0.01) ng/L vs.(16.64±0.01) ng/L,P＜0.01]화개[(2.14±0.10) mmol/L vs.(2.68±0.16)mmol/L,P＜0.01]함량현저강저,차이유통계학의의.여모형조상비,UVA조사조화UVB조사조고골근심단골밀도[(0.248±0.092) g/cm2,(0.218±0.123) g/cm2,vs.(0.162±0.125) g/cm2,P＜0.01]、중심골밀도[(0.272±0.010) g/cm2,(0.275±0.036) g/cm2,vs.(0.205±0.102) g/cm2,P＜0.01]、원심단골밀도[(0.251±0.009) g/cm2,(0.242±0.063) g/cm2,vs.(0.153±0.119) g/cm2,P＜0.01]현저승고,혈청1,25(OH)2D3[(29.47±4.54) ng/L,(27.56±6.33)ng/L,vs.(19.80±1.67)ng/L,P＜0.01]、골개소[(15.70±0.01) ng/L,(15.62±0.02) ng/L,vs.(11.00±0.01) ng/L,P ＜0.01]、개[(2.48±0.22) mmol/L,(2.58±0.13) mmol/L,vs.(2.14±0.10) mmol/L,P＜0.01]함량현저승고,차이유통계학의의.여UVB조사조상비,UVA조사조대서혈청1,25(OH)2D3、골개소、개화린함량급골밀도적차이무통계학의의.결론:UVA화UVB균능제고거란소골질소송모형대서혈청1,25(OH)2D3함량,촉진골형성,승고골밀도,완해골질소송조성적골질주실.
