CAJ | 학술논문

目的观察咳喘宁口服液对呼吸道合胞病毒诱发支气管哮喘(以下简称“哮喘”)大鼠CD4+、CD8+T细胞内磷脂酰肌醇-3-激酶(PI3K)-110α表达的影响，探讨其防治病毒诱发哮喘的机理。方法将SD大鼠随机分为磷酸盐缓冲液(PBS)对照组、模型组、地塞米松＋沙丁胺醇组(阳性药组)和咳喘宁组。建立卵白蛋白哮喘大鼠模型，并以呼吸道合胞病毒激发哮喘。采用磁珠结合法分离CD4+、CD8+T细胞，使用流式细胞仪检测T细胞内PI3K-110α的表达水平。结果模型组大鼠CD4+、CD8+T细胞百分率及CD4+、CD8+T细胞内PI3K-110α的表达水平显著高于PBS对照组、阳性药组及咳喘宁组，差异有统计学意义(均P＜0.01)。经Pearson相关分析，各组大鼠CD4+、CD8+T细胞百分率与CD4+、CD8+T细胞内表达的PI3K-110α的表达水平无明显相关性。结论 CD4+、CD8+T细胞参与了哮喘免疫平衡调节，其表达的PI3K在病毒诱发哮喘模型中明显增高。咳喘宁口服液能抑制CD4+、CD8+T细胞内PI3K的表达，可能是其治疗哮喘的免疫机制之一。
목적관찰해천저구복액대호흡도합포병독유발지기관효천(이하간칭“효천”)대서CD4+、CD8+T세포내린지선기순-3-격매(PI3K)-110α표체적영향，탐토기방치병독유발효천적궤리。방법장SD대서수궤분위린산염완충액(PBS)대조조、모형조、지새미송＋사정알순조(양성약조)화해천저조。건립란백단백효천대서모형，병이호흡도합포병독격발효천。채용자주결합법분리CD4+、CD8+T세포，사용류식세포의검측T세포내PI3K-110α적표체수평。결과모형조대서CD4+、CD8+T세포백분솔급CD4+、CD8+T세포내PI3K-110α적표체수평현저고우PBS대조조、양성약조급해천저조，차이유통계학의의(균P＜0.01)。경Pearson상관분석，각조대서CD4+、CD8+T세포백분솔여CD4+、CD8+T세포내표체적PI3K-110α적표체수평무명현상관성。결론 CD4+、CD8+T세포삼여료효천면역평형조절，기표체적PI3K재병독유발효천모형중명현증고。해천저구복액능억제CD4+、CD8+T세포내PI3K적표체，가능시기치료효천적면역궤제지일。
Objective To observe the effect of Kechuanning Oral liquid on the expression of P13K in CD4+and CD8+T cells of asthma rats induced by respiratory syncytial virus (RSV), and explore its mechanism of preventing and treating asthma induced by virus. Methods SD rats were randomly divided into phosphate-buffered saline (PBS) control group, model group, dexamethasone and salbutamol group (positive drug group), and Kechuanning oral liquid group. Ovalbumin asthma rats model was built and induced by RSV. CD4+and CD8+T cells were separated by magnetic beads method. The expressive level of PI3K-110αin T cells was detected by immumofluorescence method with flow cytometry. Results Compared with PBS control group, positive drug group and Kechuanning oral liquid group, the CD4+and CD8+T cells percentage and the expression level of PI3K-110α in CD4+ and CD8+T cells of model group were significantly higher. The differences were statistically significant (P<0.01). The CD4+and CD8+T cells percentage was not associated with the expression level of PI3K-110α in CD4+ and CD8+T cells of each group by Pearson correlation analysis. Conclusion The CD4+ and CD8+T cells involve in the asthma immunity balanced modulation, and the level of PI3K expressed by CD4+ and CD8+T cells increase distinctly. Kechuanning oral liquid can restrain the expression of PI3K in CD4+ and CD8+T cells, which might be one of the immunologic mechanisms of treating asthma.