华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2014年
2期
219-222
,共4页
法舒地尔%星形胶质细胞%肿瘤坏死因子-α%白细胞介素-8
法舒地爾%星形膠質細胞%腫瘤壞死因子-α%白細胞介素-8
법서지이%성형효질세포%종류배사인자-α%백세포개소-8
Fasudil%astrocyte%TNF-α%IL-8
目的：观察法舒地尔对星型胶质细胞分泌炎性因子TNF-α和IL-8的影响。方法原代培养SD大鼠脑星形胶质细胞，将其随机分为对照组、糖氧剥夺组（OGD组）、法舒地尔干预组3组，在培养0、3、6、12h和24h收取上层培养液，用ELISA法测定各组培养液中TNF-α和IL-8的浓度。结果与对照组相比，OGD组星形胶质细胞分泌的TNF-α和IL-8在观察时间点内明显升高（均P＜0.05），法舒地尔干预组星形胶质细胞分泌的TNF-α和IL-8也有升高，但较OGD组明显降低（均P＜0.05）。结论OGD可以刺激星形胶质细胞分泌TNF-α和IL-8，而法舒地尔能有效抑制OGD星形胶质细胞对TNF-α和IL-8的分泌，提示法舒地尔在星形胶质细胞分泌TNF-α和IL-8的调控中发挥重要作用。
目的：觀察法舒地爾對星型膠質細胞分泌炎性因子TNF-α和IL-8的影響。方法原代培養SD大鼠腦星形膠質細胞，將其隨機分為對照組、糖氧剝奪組（OGD組）、法舒地爾榦預組3組，在培養0、3、6、12h和24h收取上層培養液，用ELISA法測定各組培養液中TNF-α和IL-8的濃度。結果與對照組相比，OGD組星形膠質細胞分泌的TNF-α和IL-8在觀察時間點內明顯升高（均P＜0.05），法舒地爾榦預組星形膠質細胞分泌的TNF-α和IL-8也有升高，但較OGD組明顯降低（均P＜0.05）。結論OGD可以刺激星形膠質細胞分泌TNF-α和IL-8，而法舒地爾能有效抑製OGD星形膠質細胞對TNF-α和IL-8的分泌，提示法舒地爾在星形膠質細胞分泌TNF-α和IL-8的調控中髮揮重要作用。
목적：관찰법서지이대성형효질세포분비염성인자TNF-α화IL-8적영향。방법원대배양SD대서뇌성형효질세포，장기수궤분위대조조、당양박탈조（OGD조）、법서지이간예조3조，재배양0、3、6、12h화24h수취상층배양액，용ELISA법측정각조배양액중TNF-α화IL-8적농도。결과여대조조상비，OGD조성형효질세포분비적TNF-α화IL-8재관찰시간점내명현승고（균P＜0.05），법서지이간예조성형효질세포분비적TNF-α화IL-8야유승고，단교OGD조명현강저（균P＜0.05）。결론OGD가이자격성형효질세포분비TNF-α화IL-8，이법서지이능유효억제OGD성형효질세포대TNF-α화IL-8적분비，제시법서지이재성형효질세포분비TNF-α화IL-8적조공중발휘중요작용。
Objective To observe the effect of Fasudil on inflammatory factors (TNF-α and IL-8) secreted by astro-cytes.Methods Primary rat spinal astrocytes were cultured and randomly divided into three groups :control group ,oxygen-glu-cose deprivation group (OGD group) and Fasudil group.The culture media were harvested at different time points (0 ,3 ,6 ,12 , 24 h) for detection of the concentration of TNF-αand IL-8 by ELISA.Results Compared with the control group ,the concentra-tions of TNF-αand IL-8 in OGD group were significantly increased at each time point (P<0.05);in Fasudil group ,the concen-trations of TNF-αand IL-8 increased at some time points ,but they were much lower than in OGD group (P<0.05) .Conclusion OGD can promote the secretion of TNF-αand IL-8 by astrocytes in vitro ,which can be effectively inhibited by Fasudil.It is suggested that Fasudil plays an important role in regulating the levels of TNF-αand IL-8 secreted by astrocytes.
