CAJ | 학술논문

为了提高辣椒子叶不定芽的伸长率和遗传转化效率,本研究以保加利亚尖椒子叶为外植体,通过正交试验分别对影响保加利亚尖椒子叶不定芽伸长的激素组合以及遗传转化参数进行了优化。结果表明：诱导不定芽伸长的最佳激素组合为0.2mg/LIAA＋1.0mg/LGA3＋0.1mg/LPBU,不定芽伸长率最高为60%;以5mg/L潮霉素为选择压,预培养时间为4d、共培养时间为2d、侵染时间为20min时,诱导的抗性不定芽比率最高。本研究建立的辣椒再生及遗传转化体系为辣椒转基因研究奠定一定的基础。
위료제고랄초자협불정아적신장솔화유전전화효솔,본연구이보가리아첨초자협위외식체,통과정교시험분별대영향보가리아첨초자협불정아신장적격소조합이급유전전화삼수진행료우화。결과표명：유도불정아신장적최가격소조합위0.2mg/LIAA＋1.0mg/LGA3＋0.1mg/LPBU,불정아신장솔최고위60%;이5mg/L조매소위선택압,예배양시간위4d、공배양시간위2d、침염시간위20min시,유도적항성불정아비솔최고。본연구건립적랄초재생급유전전화체계위랄초전기인연구전정일정적기출。
In order to improve the adventitious buds elongation rate and transformation efficiency of cotyledons of Bulgaria pointed pepper,The different combinations of plant growth substance and conditions for the regeneration and genetic transformation of pepper（Capsicum annuum L.） cotyledon explants were optimized by orthogonal test.The results showed that the best combinations of orthogonal experimental design were 0.2 mg/L IAA＋1.0 mg/L GA3＋0.1 mg/L PBU and the highest rate of adventitious buds elongation was 60%.Using hygromycin at concentrations of 5 mg/L as selection pressure,the highest transformation efficiency was obtained after the explants underwent 4 days pre-culture,2 days co-culture and being infected with Agrobacterium for 20 min,The regeneration and genetic transformation system established in this research would lay the foundation for transgene researchon pepper.