CAJ | 학술논문

采用Tail-PCR和常规PCR技术首次克隆出斑鳜myostatin基因及其启动子序列。经生物信息学分析发现,斑鳜myostatin基因由3个外显子和2个内含子组成,编码区1131bp,共编码376个氨基酸。斑鳜myostatin启动子区域大小为840bp,存在1个TATAA-box、4个E-box和1个CAAT-box作用元件。利用软件CLUSTALW和MEGA3.1构建14种硬骨鱼的myostatin启动子的系统进化树结果表明：斑鳜同大口黑鲈亲缘关系最近,与鲤鱼和缨野鲮亲缘关系较远,其结果与传统形态学分类中的亲缘关系一致。斑鳜myostatin基因及其启动子克隆与特征分析,将为进一步研究鱼类myostatin基因的表达调控及其功能分析提供参考。
채용Tail-PCR화상규PCR기술수차극륭출반궐myostatin기인급기계동자서렬。경생물신식학분석발현,반궐myostatin기인유3개외현자화2개내함자조성,편마구1131bp,공편마376개안기산。반궐myostatin계동자구역대소위840bp,존재1개TATAA-box、4개E-box화1개CAAT-box작용원건。이용연건CLUSTALW화MEGA3.1구건14충경골어적myostatin계동자적계통진화수결과표명：반궐동대구흑로친연관계최근,여리어화영야릉친연관계교원,기결과여전통형태학분류중적친연관계일치。반궐myostatin기인급기계동자극륭여특정분석,장위진일보연구어류myostatin기인적표체조공급기공능분석제공삼고。
The sequences of myostatin gene and its promoter in Golden Mandarin Fish（Siniperca scherzeri）,for the first time,were cloned through the conventional PCR and Tail-PCR technology.Bioinformatics analysis shows that the myostatin gene consists of three exons and two introns and the coding region is 1 131 bp encoding 376 amino acid residues.The nucleotide sequence analysis shows that myostatin promoter is 840 bp and contains several regulatory elements,such as one TATAA-box,four E-boxes and one CAAT-box.The phylogenetic tree of the myostatin gene promoters from 14 teleost species was constructed with CLUSTALW and MEGA3.1.The result indicates that Golden Mandarin Fish（Siniperca scherzeri） has a closer phylogenetic relationship with Largemouth bass（Micropterus salmoides）,but it is far from Cyprinus carpio and Labeo fimbriatus.This result is consistent with general morphological taxonomy.This study would contribute to the further research on the regulation of myostatin expression and the function of myostatin in teleost fish.