实用检验医师杂志
實用檢驗醫師雜誌
실용검험의사잡지
Chinese Journal of Laboratory Pathologist
2014年
3期
173-175
,共3页
EB病毒相关性噬血细胞综合征%T淋巴细胞%CD69%儿童
EB病毒相關性噬血細胞綜閤徵%T淋巴細胞%CD69%兒童
EB병독상관성서혈세포종합정%T림파세포%CD69%인동
Epstein-barr virus associated hemophagocytic syndrome%T lymphocyte%CD69%Chil-dren
目的:探讨外周血T淋巴细胞CD69表达在EB病毒相关性噬血细胞综合征(epstein-barr virus-associated hemophagocytic syndrome, EBV-AHS)患儿中的变化及意义。方法应用流式细胞术检测EBV-AHS患儿(急性期28例,缓解期21例)及30例健康对照儿童外周血CD3+CD4+CD69+及CD3+CD8+CD69+的表达水平,对检测结果进行统计学分析。结果三组受试者间CD3+CD4+CD69+及CD3+CD8+CD69+的表达水平差异均有统计学意义(P均<0.01);急性期组CD3+CD4+CD69+及CD3+CD8+CD69+的表达水平明显高于缓解期组及对照组,且差异均有统计学意义(P均<0.01);缓解期组CD3+CD4+CD69+及CD3+CD8+CD69+的表达水平明显高于对照组,且差异均有统计学意义(P均<0.01)。结论 T淋巴细胞的过度活化在EBV-AHS发病过程中起重要作用,是EBV-AHS重要的发病机理之一。检测T淋巴细胞CD69的表达有利于临床判断治疗效果,并为应用免疫调节剂提供理论依据。
目的:探討外週血T淋巴細胞CD69錶達在EB病毒相關性噬血細胞綜閤徵(epstein-barr virus-associated hemophagocytic syndrome, EBV-AHS)患兒中的變化及意義。方法應用流式細胞術檢測EBV-AHS患兒(急性期28例,緩解期21例)及30例健康對照兒童外週血CD3+CD4+CD69+及CD3+CD8+CD69+的錶達水平,對檢測結果進行統計學分析。結果三組受試者間CD3+CD4+CD69+及CD3+CD8+CD69+的錶達水平差異均有統計學意義(P均<0.01);急性期組CD3+CD4+CD69+及CD3+CD8+CD69+的錶達水平明顯高于緩解期組及對照組,且差異均有統計學意義(P均<0.01);緩解期組CD3+CD4+CD69+及CD3+CD8+CD69+的錶達水平明顯高于對照組,且差異均有統計學意義(P均<0.01)。結論 T淋巴細胞的過度活化在EBV-AHS髮病過程中起重要作用,是EBV-AHS重要的髮病機理之一。檢測T淋巴細胞CD69的錶達有利于臨床判斷治療效果,併為應用免疫調節劑提供理論依據。
목적:탐토외주혈T림파세포CD69표체재EB병독상관성서혈세포종합정(epstein-barr virus-associated hemophagocytic syndrome, EBV-AHS)환인중적변화급의의。방법응용류식세포술검측EBV-AHS환인(급성기28례,완해기21례)급30례건강대조인동외주혈CD3+CD4+CD69+급CD3+CD8+CD69+적표체수평,대검측결과진행통계학분석。결과삼조수시자간CD3+CD4+CD69+급CD3+CD8+CD69+적표체수평차이균유통계학의의(P균<0.01);급성기조CD3+CD4+CD69+급CD3+CD8+CD69+적표체수평명현고우완해기조급대조조,차차이균유통계학의의(P균<0.01);완해기조CD3+CD4+CD69+급CD3+CD8+CD69+적표체수평명현고우대조조,차차이균유통계학의의(P균<0.01)。결론 T림파세포적과도활화재EBV-AHS발병과정중기중요작용,시EBV-AHS중요적발병궤리지일。검측T림파세포CD69적표체유리우림상판단치료효과,병위응용면역조절제제공이론의거。
Objective To explore the change and significance of the peripheral blood T lymphocyte CD69 expression in children patients with epstein-barr virus-associated hemophagocytic syndrome (EBV-AHS). Methods The expression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in EBV-AHS children(28 cases in acute phase and 21 cases in paracmasis) and 30 cases of healthy controls were detected by flow cy-tometry method. The results were analyzed statistically. Results There were statistical significance in the dif-ferences of CD3+CD4+CD69+and CD3+CD8+CD69+expression levels among three groups(Pall<0.01). The ex-pression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in acute phase group were all higher than that of paracmasis group and control group, and the differences all had statistiacal significance (Pall<0.01). The ex-pression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in paracmasis group were all higher than that of con-trol group, and the differences all had statistiacal significance (Pall<0.01). Conclusion Excessively activate of T lymphocyte play an important role in the EBV-AHS attack procession. Detection of T lymphocyte CD69 expression is helpful for treatment effect judgment in clinical , and provide theoretical basis for application of immune modulators.