实用检验医师杂志
實用檢驗醫師雜誌
실용검험의사잡지
Chinese Journal of Laboratory Pathologist
2014年
3期
147-151,152
,共6页
宫颈癌细胞%多肽N-乙酰氨基半乳糖转移酶7%RNA干扰
宮頸癌細胞%多肽N-乙酰氨基半乳糖轉移酶7%RNA榦擾
궁경암세포%다태N-을선안기반유당전이매7%RNA간우
Cervical cancer cell%Polypeptide N-acetylgalactosaminyltransferase 7%RNA interfer-ence
目的：探讨多肽N-乙酰氨基半乳糖转移酶7（polypeptide N-acetylgalactosaminyltransfer-ase 7， GALNT7）基因对宫颈癌细胞生长、侵袭及迁移能力的影响。方法利用RNA干扰技术沉默GALNT7（siR-GALNT7），以非特异性序列（pSilencer 2．1）转染细胞作为阴性对照，转染宫颈癌细胞HeLa和C33A细胞，采用MTT实验、集落形成实验、Transwell侵袭实验以及细胞划痕实验来检测干扰GAL-NT7基因后宫颈癌细胞生长、侵袭以及转移能力的变化。结果 Western Blot检测结果显示，转染siR-GALNT7质粒的实验组中两种宫颈癌细胞中GALNT7的蛋白表达水平明显降低，与转染siR-Ctrl的对照组比较，差异均有统计学意义（P均＜0．05）；siR-GALNT7使HeLa和C33A细胞生长活性分别下降了19％和22％，与对照组比较，差异均有统计学意义（P均＜0．05）；siR-GALNT7使HeLa和C33A细胞的集落形成能力分别下降了56％和52％，与对照组比较，差异均有统计学意义（P均＜0．05）；siR-GALNT7使HeLa和C33A细胞侵袭能力分别下降了48％和54％，与对照组比较，差异均有统计学意义（P均＜0．05）；siR-GALNT7使HeLa细胞迁移能力下降了约30％，与对照组比较差异有统计学意义（P＜0．05）。结论 RNA干扰在体外明显降低了宫颈癌细胞中GALNT7基因的表达，同时抑制了宫颈癌细胞的生长侵袭以及迁移能力，GALNT7基因有可能成为宫颈癌基因治疗的重要靶点。
目的：探討多肽N-乙酰氨基半乳糖轉移酶7（polypeptide N-acetylgalactosaminyltransfer-ase 7， GALNT7）基因對宮頸癌細胞生長、侵襲及遷移能力的影響。方法利用RNA榦擾技術沉默GALNT7（siR-GALNT7），以非特異性序列（pSilencer 2．1）轉染細胞作為陰性對照，轉染宮頸癌細胞HeLa和C33A細胞，採用MTT實驗、集落形成實驗、Transwell侵襲實驗以及細胞劃痕實驗來檢測榦擾GAL-NT7基因後宮頸癌細胞生長、侵襲以及轉移能力的變化。結果 Western Blot檢測結果顯示，轉染siR-GALNT7質粒的實驗組中兩種宮頸癌細胞中GALNT7的蛋白錶達水平明顯降低，與轉染siR-Ctrl的對照組比較，差異均有統計學意義（P均＜0．05）；siR-GALNT7使HeLa和C33A細胞生長活性分彆下降瞭19％和22％，與對照組比較，差異均有統計學意義（P均＜0．05）；siR-GALNT7使HeLa和C33A細胞的集落形成能力分彆下降瞭56％和52％，與對照組比較，差異均有統計學意義（P均＜0．05）；siR-GALNT7使HeLa和C33A細胞侵襲能力分彆下降瞭48％和54％，與對照組比較，差異均有統計學意義（P均＜0．05）；siR-GALNT7使HeLa細胞遷移能力下降瞭約30％，與對照組比較差異有統計學意義（P＜0．05）。結論 RNA榦擾在體外明顯降低瞭宮頸癌細胞中GALNT7基因的錶達，同時抑製瞭宮頸癌細胞的生長侵襲以及遷移能力，GALNT7基因有可能成為宮頸癌基因治療的重要靶點。
목적：탐토다태N-을선안기반유당전이매7（polypeptide N-acetylgalactosaminyltransfer-ase 7， GALNT7）기인대궁경암세포생장、침습급천이능력적영향。방법이용RNA간우기술침묵GALNT7（siR-GALNT7），이비특이성서렬（pSilencer 2．1）전염세포작위음성대조，전염궁경암세포HeLa화C33A세포，채용MTT실험、집락형성실험、Transwell침습실험이급세포화흔실험래검측간우GAL-NT7기인후궁경암세포생장、침습이급전이능력적변화。결과 Western Blot검측결과현시，전염siR-GALNT7질립적실험조중량충궁경암세포중GALNT7적단백표체수평명현강저，여전염siR-Ctrl적대조조비교，차이균유통계학의의（P균＜0．05）；siR-GALNT7사HeLa화C33A세포생장활성분별하강료19％화22％，여대조조비교，차이균유통계학의의（P균＜0．05）；siR-GALNT7사HeLa화C33A세포적집락형성능력분별하강료56％화52％，여대조조비교，차이균유통계학의의（P균＜0．05）；siR-GALNT7사HeLa화C33A세포침습능력분별하강료48％화54％，여대조조비교，차이균유통계학의의（P균＜0．05）；siR-GALNT7사HeLa세포천이능력하강료약30％，여대조조비교차이유통계학의의（P＜0．05）。결론 RNA간우재체외명현강저료궁경암세포중GALNT7기인적표체，동시억제료궁경암세포적생장침습이급천이능력，GALNT7기인유가능성위궁경암기인치료적중요파점。
Objective To investigate the influence of polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) gene to growth, invasiveness and migration of cervical cancer cells. Methods GALNT7 gene was silenced by RNA interference, took non-specificity sequence pSilencer 2.1 as negative control. After transfection of cervical cancer cells (HeLa and C33A cells), Western Blot was used to detect protein expres-sion of GALNT7 gene, the growth ability was detected by MTT assay, the ability to form colonies was evaluated by the colony formation assay, the invasion and migration ability were detected by matrigel invasion assay. Re-sults In experimental group the protein expression of GALNT7 was decreased significantly than that of control group, and the differences all had statistical significance(Pall<0.05). MTT assay revealed that cell growth was inhibited, the inhibitory rates were decreased by19% in HeLa cells and 22% in C33A cells, and compared with control group, the differences all had statistical significance (Pall<0.05). The ability to form colonies in experimental group were inhibited by 56%in HeLa cells and 52%in C33A cells than that of control group , and the differences all had statistical significance(Pall<0.05). In experimental group, the inhibitory rates on inva-sion were decreased by 48%in HeLa cells and 54%in C33A cells , and the inhibitory rate on migration was decreased by 30%in HeLa cells, and compared with control group, the differences all had statistical signifi-cance (Pall<0.05). Conclusion RNAi mediated GALNT7 down-regulation can inhibit the growth, invasion and migration of cervical cancer, and can be an important target to cervical cancer gene-therapies.
