CAJ | 학술논문

利用环烷酸、二乙烯三胺或三乙烯四胺、硫酸二乙酯为原料合成了2种咪唑啉季铵盐M1和M2，M1，M2分别在pH＝2．36和pH＝3．78的Britton-Robinson缓冲溶液中与fsDNA相互作用后，体系的共振光散射（RLS）强度明显增强．研究了它们与fsDNA作用的影响因素，在优化条件下，RLS强度增加值与fsDNA浓度之间呈良好的线性关系，拟定了新的测定fsDNA的RLS法．该法具有灵敏度高，操作简单，准确度好，线性范围宽等特点，用于合成样的测定，结果满意．
이용배완산、이을희삼알혹삼을희사알、류산이을지위원료합성료2충미서람계안염M1화M2，M1，M2분별재pH＝2．36화pH＝3．78적Britton-Robinson완충용액중여fsDNA상호작용후，체계적공진광산사（RLS）강도명현증강．연구료타문여fsDNA작용적영향인소，재우화조건하，RLS강도증가치여fsDNA농도지간정량호적선성관계，의정료신적측정fsDNA적RLS법．해법구유령민도고，조작간단，준학도호，선성범위관등특점，용우합성양적측정，결과만의．
Two imidazolinyl-quaternary-ammonium-salt M1 and M2 were synthesised using naphthenic acid, diethylene triamine (or triethylene tetramine),and diethyl sulfate.The interaction between fsDNA respectively with M1 and M2 ,enhanced the intensities of resonance light scattering (RLS)significantly in Britton- Robinson buffer solution of the pH 2.36 for M1 and 3.78 for M2.Also,it was studied the effect factors on the reaction between imidazolinyl-quaternary-ammonium-salt and fsDNA. Under the optimum conditions, the enhanced intensities of RLS had good linear relationship with the concentration of fsDNA.Thus,a new RLS method for determination of fsDNA was established.The method has the properties of high sensitivity,simple operation, good accuracy and the wide range of linear.It can be used to the determination of fsDNA contain in the synthesized samples with the result of satisfying.