CAJ | 학술논문

目的：研究Axl 基因在喉癌细胞Hep-2中的表达，探讨Axl 基因与喉癌细胞的增值、浸润及转移的关系。方法提取Hep-2和人永生化表皮细胞Hacat细胞RNA，应用RT-PCR检测Axl mRNA的表达；应用免疫细胞化学染色检测Axl蛋白质在细胞Hep-2和Hacat中的表达。结果 AxlmRNA在Hep-2细胞中高表达，平均相对积分吸光密度值为：0．8601±0．0409；Axl mRNA 在 Hacat 细胞中低表达，平均相对积分吸光密度值为：0．2637±0.0503，差异有统计学意义（t＝18．682，P＜0．01）。免疫细胞化学染色表明 Axl在 Hep-2细胞中高表达，平均吸光密度值为：0．2621±0．0238；在 Hacat细胞中低表达，平均吸光密度为：0．0729±0．0201，差异有统计学意义（t ＝25.938，P＜0.01）。结论 Axl与喉癌细胞Hep-2的增值、浸润及转移有密切关系。
목적：연구Axl 기인재후암세포Hep-2중적표체，탐토Axl 기인여후암세포적증치、침윤급전이적관계。방법제취Hep-2화인영생화표피세포Hacat세포RNA，응용RT-PCR검측Axl mRNA적표체；응용면역세포화학염색검측Axl단백질재세포Hep-2화Hacat중적표체。결과 AxlmRNA재Hep-2세포중고표체，평균상대적분흡광밀도치위：0．8601±0．0409；Axl mRNA 재 Hacat 세포중저표체，평균상대적분흡광밀도치위：0．2637±0.0503，차이유통계학의의（t＝18．682，P＜0．01）。면역세포화학염색표명 Axl재 Hep-2세포중고표체，평균흡광밀도치위：0．2621±0．0238；재 Hacat세포중저표체，평균흡광밀도위：0．0729±0．0201，차이유통계학의의（t ＝25.938，P＜0.01）。결론 Axl여후암세포Hep-2적증치、침윤급전이유밀절관계。
Objective To study the expression of Anexelekto (Axl)in human laryngeal carcinoma cellsand its significance. Methods In this study,we investigated the expression of Axl mRNA in humanlaryngeal carcinoma cells,Hep-2 cells,using techniques of RT-PCR,and investigated the expression of Axlin the Hep-2 cells using techniques of immunohistochemistry. Results It showed that the expression levelof Axl and Axl mRNA significantly increased in Hep-2 cells as compared with the normal cells,Hacat cells(P <0.01 ). Conclusion These data raise the possibility that Axl many play key roles in the growth,invasion and metastasis of human laryngeal carcinoma.