淮北师范大学学报（自然科学版）
淮北師範大學學報（自然科學版）
회북사범대학학보（자연과학판）
JOURNAL OF HUAIBEI COAL INDUSTRY TEACHERS COLLEGE(NATURAL SCIENCE)
2014年
3期
38-41
,共4页
维生素K3%三聚氰胺%荧光猝灭
維生素K3%三聚氰胺%熒光猝滅
유생소K3%삼취청알%형광졸멸
vitamin K3%melamine%fluorescence quenching
在pH 7.0的缓冲体系中，维生素K3使得三聚氰胺的荧光发生明显猝灭.在激发波长为256 nm，发射波长为366 nm处，三聚氰胺荧光猝灭程度在一定范围内与维生素K3的浓度成正比，从而建立了荧光猝灭法测定痕量维生素K3的新方法.方法的线性范围为0.10-10.00 mg/L，检出限为0.085 mg/L，方法已应用于实际样品的测定.
在pH 7.0的緩遲體繫中，維生素K3使得三聚氰胺的熒光髮生明顯猝滅.在激髮波長為256 nm，髮射波長為366 nm處，三聚氰胺熒光猝滅程度在一定範圍內與維生素K3的濃度成正比，從而建立瞭熒光猝滅法測定痕量維生素K3的新方法.方法的線性範圍為0.10-10.00 mg/L，檢齣限為0.085 mg/L，方法已應用于實際樣品的測定.
재pH 7.0적완충체계중，유생소K3사득삼취청알적형광발생명현졸멸.재격발파장위256 nm，발사파장위366 nm처，삼취청알형광졸멸정도재일정범위내여유생소K3적농도성정비，종이건립료형광졸멸법측정흔량유생소K3적신방법.방법적선성범위위0.10-10.00 mg/L，검출한위0.085 mg/L，방법이응용우실제양품적측정.
A new fluorescence quenching method was developed for the determination of vitamin K3 based on its notable quenching effect on the fluorescence of melamine in pH 7.0 buffer medium.The fluorescence quenching value of melamine was proportional to the concentration of vitamin K3 in a certain range.The excita-tion and emission wavelengths were 256 nm and 366 nm,respectively.The linear range for the determination of vitamin K3 is 0.10-10.00 mg/L.The detection limit is 0.085 mg/L.The method has been applied in the de-termination of vitamin K3 in real samples with satisfactory results.
