山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
22期
14-16
,共3页
郁翠萍%李美加%张向宇%杨文华
鬱翠萍%李美加%張嚮宇%楊文華
욱취평%리미가%장향우%양문화
橙皮苷%氟化钠%牙本质%牛恒牙%再矿化
橙皮苷%氟化鈉%牙本質%牛恆牙%再礦化
등피감%불화납%아본질%우항아%재광화
hesperidin%sodium fluoride%dentin%permanent bovine teeth%remineralization
目的：探讨橙皮苷对牛牙根部牙本质再矿化的影响。方法取新鲜拔除的年轻牛恒牙26颗，高速硬组织切割机将牙根切成6 mm ×6 mm ×2 mm的牙本质切片，随机分为研究组、阳性对照组及空白对照组，每组6个切片。将各组标本浸入100 mL脱矿液中浸泡96 h，使牙本质形成脱矿病变。将研究组、阳性对照组及空白对照组切片分别放入5000 mg/L橙皮苷、1000 mg/L氟化钠及去离子水中浸泡2 h，各组切片放入再矿化液中8 h，连续循环8 d。收集整个循环过程中的再矿化液及各组牙本质切片，用羟脯氨酸法检测各组再矿化液中的羟脯氨酸含量，X线能谱分析法检测牙本质Ca、P含量。结果研究组、阳性对照组及空白对照组再矿化液羟脯氨酸含量分别为（22．80±11．78）、（16．43±7．87）、（64．16±3．92），研究组、阳性对照组再矿化液中羟脯氨酸含量均高于空白对照组（P＜0．05）。研究组、阳性对照组牙本质Ca/P比例高于空白对照组，研究组低于阳性对照组（P均＜0．05）。结论在体外条件下，橙皮苷具有抑制牛牙根部牙本质胶原降解、促进脱矿牙本质再矿化的作用。
目的：探討橙皮苷對牛牙根部牙本質再礦化的影響。方法取新鮮拔除的年輕牛恆牙26顆，高速硬組織切割機將牙根切成6 mm ×6 mm ×2 mm的牙本質切片，隨機分為研究組、暘性對照組及空白對照組，每組6箇切片。將各組標本浸入100 mL脫礦液中浸泡96 h，使牙本質形成脫礦病變。將研究組、暘性對照組及空白對照組切片分彆放入5000 mg/L橙皮苷、1000 mg/L氟化鈉及去離子水中浸泡2 h，各組切片放入再礦化液中8 h，連續循環8 d。收集整箇循環過程中的再礦化液及各組牙本質切片，用羥脯氨痠法檢測各組再礦化液中的羥脯氨痠含量，X線能譜分析法檢測牙本質Ca、P含量。結果研究組、暘性對照組及空白對照組再礦化液羥脯氨痠含量分彆為（22．80±11．78）、（16．43±7．87）、（64．16±3．92），研究組、暘性對照組再礦化液中羥脯氨痠含量均高于空白對照組（P＜0．05）。研究組、暘性對照組牙本質Ca/P比例高于空白對照組，研究組低于暘性對照組（P均＜0．05）。結論在體外條件下，橙皮苷具有抑製牛牙根部牙本質膠原降解、促進脫礦牙本質再礦化的作用。
목적：탐토등피감대우아근부아본질재광화적영향。방법취신선발제적년경우항아26과，고속경조직절할궤장아근절성6 mm ×6 mm ×2 mm적아본질절편，수궤분위연구조、양성대조조급공백대조조，매조6개절편。장각조표본침입100 mL탈광액중침포96 h，사아본질형성탈광병변。장연구조、양성대조조급공백대조조절편분별방입5000 mg/L등피감、1000 mg/L불화납급거리자수중침포2 h，각조절편방입재광화액중8 h，련속순배8 d。수집정개순배과정중적재광화액급각조아본질절편，용간포안산법검측각조재광화액중적간포안산함량，X선능보분석법검측아본질Ca、P함량。결과연구조、양성대조조급공백대조조재광화액간포안산함량분별위（22．80±11．78）、（16．43±7．87）、（64．16±3．92），연구조、양성대조조재광화액중간포안산함량균고우공백대조조（P＜0．05）。연구조、양성대조조아본질Ca/P비례고우공백대조조，연구조저우양성대조조（P균＜0．05）。결론재체외조건하，등피감구유억제우아근부아본질효원강해、촉진탈광아본질재광화적작용。
Objective To investigate the effect of hesperidin on the remineralization process of bovine root dentine in vitro.Methods Twenty-six extracted young permanent bovine teeth were prepared into 6 mm ×6 mm ×2 mm size of den-tin slices, and randomly divided into3 groups:the research group, positive control group and blank control group , six slices in each group.Specimens were immersed in demineralizing solution for 96 hours, and then we formed dentin demineraliza-tion lesions.Specimens were incubated in testing solutions (5 000 mg/L hesperidin, 1 000 mg/L NaF and deionized wa-ter) for 2 hours and remineralizing solution for 8 hours, for 8 days.The remineralizing solution and dentin slices of each group were collected .Hydroxyproline content of the remineralizing solution was investigated by hydroxyproline assay , den-tine Ca and P contents were analyzed by energy-dispersive X-ray spectrometry .Results Hydroxyproline contents of the research group, positive control group and blank control group in the remineralizing solution were respectively (22.80 ± 11.78), (16.43 ±7.87) and (64.16 ±3.92).Hydroxyproline contents of the research group and the positive control group were higher than that of the blank control group (P<0.05).Dentine Ca/P ratios of the research group and the posi-tive control group were higher than that of the blank control group , the research group was lower than the positive control group (all P<0.05).Conclusion Hesperidin inhibits dentine collagen degradation and enhanced remineralization in vitro.
