CAJ | 학술논문

目的：观察牛蒡子苷元对鸡胚绒毛尿囊膜（ CAM）血管新生的影响。方法采用乙醇结晶法制备牛蒡子苷元，开窗法制备CAM模型。将CAM模型中的40只鸡胚随机分为阴性对照组、低剂量组、中剂量组、高剂量组，每组各10只。分别将含有DMSO、2．5、5．0、10．0 g/L牛蒡子苷元的药物载体置于阴性对照组、低剂量组、中剂量组、高剂量组CAM中央血管稀少区，孵育48 h后，剪下CAM，在解剖显微镜下观察药物载体覆盖区及CAM周围的新生血管数量、形态，观察各组新生血管等级，记录各组药物载体边缘5 mm内新生血管的数目，计算血管抑制率（ IR）。结果低、中、高剂量组CAM新生血管发育均受抑制。中、高剂量组新生血管等级3＋、4＋的数量多于阴性对照组（P＜0．05），阴性对照组、低剂量组、中剂量组、高剂量组新生血管数目分别为（78．26±5．62）、（59．22±4．16）、（37．43±6．51）、（24．17±3．23）条，低剂量组新生血管数低于阴性对照组（P＜0．05），中、高剂量组新生血管数均低于阴性对照组（P＜0．01），且中、高剂量组新生血管数均低于低剂量组（P＜0．05）。低剂量组、中剂量组、高剂量组新生血管IR分别为24．33％、52．17％及69．12％。结论牛蒡子苷元具有抑制鸡胚CAM血管新生的作用。
목적：관찰우방자감원대계배융모뇨낭막（ CAM）혈관신생적영향。방법채용을순결정법제비우방자감원，개창법제비CAM모형。장CAM모형중적40지계배수궤분위음성대조조、저제량조、중제량조、고제량조，매조각10지。분별장함유DMSO、2．5、5．0、10．0 g/L우방자감원적약물재체치우음성대조조、저제량조、중제량조、고제량조CAM중앙혈관희소구，부육48 h후，전하CAM，재해부현미경하관찰약물재체복개구급CAM주위적신생혈관수량、형태，관찰각조신생혈관등급，기록각조약물재체변연5 mm내신생혈관적수목，계산혈관억제솔（ IR）。결과저、중、고제량조CAM신생혈관발육균수억제。중、고제량조신생혈관등급3＋、4＋적수량다우음성대조조（P＜0．05），음성대조조、저제량조、중제량조、고제량조신생혈관수목분별위（78．26±5．62）、（59．22±4．16）、（37．43±6．51）、（24．17±3．23）조，저제량조신생혈관수저우음성대조조（P＜0．05），중、고제량조신생혈관수균저우음성대조조（P＜0．01），차중、고제량조신생혈관수균저우저제량조（P＜0．05）。저제량조、중제량조、고제량조신생혈관IR분별위24．33％、52．17％급69．12％。결론우방자감원구유억제계배CAM혈관신생적작용。
Objective To observe the influence of arctigenin on the angiogenesis of chick embryo chorioallantoic membrane ( CAM) .Methods Arctigenin was prepared by the procedure of ethanol crystallization from Fructus Arctii and we used the windowing method to prepare the CAM model .Forty chick embryos of CAM models were randomly divided into the negative control group, low (2.5 g/L), medium (5.0 g/L), and high (10.0 g/L) doses of arctigenin groups, 10 em-bryos in each group .Then the negative control solution containing DMSO and each dose of liquid containing arctigenin were added respectively onto the surface of the carrier CAM .The embryo CAM model was prepared after 8-day incubation .CAM sample was cut to observe the serum angiogenesis in the drug carrier area , to count the number of new blood vessels , and to observe the levels of new blood vessels under dissecting microscope , meanwhile , the number of new blood vessels in each drug groups within 5 mm of carrier edge was recorded , and vascular inhibition rate ( IR) was calculated .Results The angiogenesis of CAM in low , medium and high doses of arctigenin groups was inhibited , the 3+, 4+number of the medi-um and high doses of arctigenin groups was more than that of the negative control group (P<0.05).The numbers of new blood vessels in the negative control group , high dose , medium dose and low dose group were respectively ( 78 .26 ± 5.62), (59.22 ±4.16), (37.43 ±6.51) and (24.17 ±3.23).The neovascularization number of low dose group was less than that of the negative control group (P<0.05), medium and high dose groups were less than that of the negative control group (P<0.01) and that of the low dose group (P<0.05).The neovascular IR of low, medium and high dose groups were 24.33%, 52.17%and 69.12%, respectively.Conclusion Arctigenin can inhibit the angiogenesis of CAM .