CAJ | 학술논문

目的：研究自发的低分化鼻咽癌细胞（ CNE-2Z）氯电流特征。方法采用全细胞膜片钳记录自发的CNE-2Z细胞氯电流，通过去除电极内液中的ATP、细胞外灌流氯离子通道阻断剂或高渗液等方法观察并分析电流的特性。结果细胞处于等渗环境中可自发激活一个具有明显外向优势的电流，该电流没有明显的时间依赖性失活，电流的翻转电位为（-7．6±0．9） mV，接近氯离子平衡电位（-0．9 mV）。该电流的激活依赖于细胞内ATP。氯通道阻断剂ATP可抑制该电流，且其对外向电流的抑制作用大于内向电流。细胞外灌流高渗液对该电流有明显抑制作用。结论 CNE-2Z细胞处于等渗环境中可自发激活一个电流特征与容积敏感性氯通道相似的氯通道。
목적：연구자발적저분화비인암세포（ CNE-2Z）록전류특정。방법채용전세포막편겸기록자발적CNE-2Z세포록전류，통과거제전겁내액중적ATP、세포외관류록리자통도조단제혹고삼액등방법관찰병분석전류적특성。결과세포처우등삼배경중가자발격활일개구유명현외향우세적전류，해전류몰유명현적시간의뢰성실활，전류적번전전위위（-7．6±0．9） mV，접근록리자평형전위（-0．9 mV）。해전류적격활의뢰우세포내ATP。록통도조단제ATP가억제해전류，차기대외향전류적억제작용대우내향전류。세포외관류고삼액대해전류유명현억제작용。결론 CNE-2Z세포처우등삼배경중가자발격활일개전류특정여용적민감성록통도상사적록통도。
Objective To explore the spontaneous chloride currents in poorly differentiated nasopharyngeal carcinoma ( CNE-2Z ) cells.Methods The whole-cell patch-clamp technique was used to detect the Cl -currents.The characteris-tics of the currents were clarified by ATP depletion in the pipette solution , extracellular applications of chloride channel blockers and hypertonic challenges .Results A chloride current was activated spontaneously and occasionally in the iso-tonic solution in CNE-2Z cells, showing significant outward rectification but no time-dependent inactivation .The reversal potential of the current was (-7.6 ±0.9) mV, which was close to the calculated equilibrium potential for Cl -.The in-duction of the chloride channel was dependent on the intracellular ATP .The spontaneous chloride currents were inhibited by extracelluar applications of the chloride channel blocker ATP and 47% hypertonic solution .Conclusion A chloride channel can be activated spontaneously and occasionally under the isotonic condition in CNE -2Z cells, showing the similar characteristics to the volume-sensitive chloride channel .