山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
24期
16-18
,共3页
买热艳木·艾尔肯%热沙来提·阿不都瓦衣特%王岩蛟%依马木·买买提明%日斯拉特·艾力木%斯坎德尔·白克力
買熱豔木·艾爾肯%熱沙來提·阿不都瓦衣特%王巖蛟%依馬木·買買提明%日斯拉特·艾力木%斯坎德爾·白剋力
매열염목·애이긍%열사래제·아불도와의특%왕암교%의마목·매매제명%일사랍특·애력목%사감덕이·백극력
肝肿瘤%原发性肝细胞癌%二乙基亚硝胺%信号转导和转录活化因子3%细胞周期素D1%大鼠
肝腫瘤%原髮性肝細胞癌%二乙基亞硝胺%信號轉導和轉錄活化因子3%細胞週期素D1%大鼠
간종류%원발성간세포암%이을기아초알%신호전도화전록활화인자3%세포주기소D1%대서
liver neoplasms%primary hepatocellular carcinoma%diethylnitrosamine%signal transducer and activator of transcription 3%cyclin D1%rats
目的：观察信号转导和转录活化因子3（STAT3）和细胞周期素D1（Cyclin D1）在大鼠肝癌组织中的表达变化，并探讨其意义。方法将72只大鼠随机分为模型组和对照组各36只，模型组自由饮用0．1 mg/mL的二乙基亚硝胺（ DEN）溶液，对照组饮用等量灭菌蒸馏水，连续饮用20周。处死大鼠取癌组织，用RT-PCR法检测STAT3、Cyclin D1 mRNA，Western blot法和免疫组化法检测STAT3、Cyclin D1蛋白。结果对照组STAT3、Cyclin D1 mRNA表达水平分别为0．32±0．12、0．18±0．05，模型组分别为0．72±0．25、0．57±0．15，P均＜0．05。 Western blot法检测模型组STAT3、Cyclin D1蛋白表达量分别为0．58±0．25、0．65±0．14，对照组分别为0．32±0．19、0．41±0．15，P均＜0．05。免疫组化法检测模型组STAT3、Cyclin D1蛋白IOD分别为2373．87±258．79、668．44±63．10，对照组分别为487．81±55．31和138．86±31．50，P均＜0．05。结论 STAT3、Cyclin D1在肝癌大鼠癌组织中表达上调，二者可促进肝癌的发生和发展。
目的：觀察信號轉導和轉錄活化因子3（STAT3）和細胞週期素D1（Cyclin D1）在大鼠肝癌組織中的錶達變化，併探討其意義。方法將72隻大鼠隨機分為模型組和對照組各36隻，模型組自由飲用0．1 mg/mL的二乙基亞硝胺（ DEN）溶液，對照組飲用等量滅菌蒸餾水，連續飲用20週。處死大鼠取癌組織，用RT-PCR法檢測STAT3、Cyclin D1 mRNA，Western blot法和免疫組化法檢測STAT3、Cyclin D1蛋白。結果對照組STAT3、Cyclin D1 mRNA錶達水平分彆為0．32±0．12、0．18±0．05，模型組分彆為0．72±0．25、0．57±0．15，P均＜0．05。 Western blot法檢測模型組STAT3、Cyclin D1蛋白錶達量分彆為0．58±0．25、0．65±0．14，對照組分彆為0．32±0．19、0．41±0．15，P均＜0．05。免疫組化法檢測模型組STAT3、Cyclin D1蛋白IOD分彆為2373．87±258．79、668．44±63．10，對照組分彆為487．81±55．31和138．86±31．50，P均＜0．05。結論 STAT3、Cyclin D1在肝癌大鼠癌組織中錶達上調，二者可促進肝癌的髮生和髮展。
목적：관찰신호전도화전록활화인자3（STAT3）화세포주기소D1（Cyclin D1）재대서간암조직중적표체변화，병탐토기의의。방법장72지대서수궤분위모형조화대조조각36지，모형조자유음용0．1 mg/mL적이을기아초알（ DEN）용액，대조조음용등량멸균증류수，련속음용20주。처사대서취암조직，용RT-PCR법검측STAT3、Cyclin D1 mRNA，Western blot법화면역조화법검측STAT3、Cyclin D1단백。결과대조조STAT3、Cyclin D1 mRNA표체수평분별위0．32±0．12、0．18±0．05，모형조분별위0．72±0．25、0．57±0．15，P균＜0．05。 Western blot법검측모형조STAT3、Cyclin D1단백표체량분별위0．58±0．25、0．65±0．14，대조조분별위0．32±0．19、0．41±0．15，P균＜0．05。면역조화법검측모형조STAT3、Cyclin D1단백IOD분별위2373．87±258．79、668．44±63．10，대조조분별위487．81±55．31화138．86±31．50，P균＜0．05。결론 STAT3、Cyclin D1재간암대서암조직중표체상조，이자가촉진간암적발생화발전。
Objective To observe the expression changes of signal transducer and activator of transcription 3 (STAT3) and cyclin D1 in hepatocellular carcinoma tissues of rats and to investigate its significance .Methods Seventy-two rats were randomly divided into the model group and control group , 36 rats in each group .The model group received 0.1 mg/mL diethylnitrosamine (DEN) freely, and the control group was given the same volume of sterile distilled water for 20 weeks.The STAT3 and cyclin D1 mRNA was detected by using RT-PCR, Western blotting and immunohistochemistry were used to detect the STAT 3 and cyclin D1 protein expression .Results In control group , the mRNA expression of STAT3 and cyclin D1 were respectively 0.32 ±0.12 and 0.18 ±0.05, in the model group respectively 0.72 ±0.25 and 0.57 ±0.15, all P<0.05;in the control group, the protein expression levels of STAT3 and cyclin D1 were respectively 0.32 ±0.19 and 0.41 ±0.15, while in the model group , they were respectively 0.58 ±0.25 and 0.65 ±0.14, all P<0.05;immunohistochemical results showed that the IOD of STAT 3 and cyclin D1 in the model group was respectively 2 373.87 ±258.79 and 668.44 ±63.10, in control group, it was respectively 487.81 ±55.31 and 138.86 ±31.50, all P<0.05.Conclusion The expression of STAT3 and cyclin D1 in the hepatocellular carcinoma tissues of rats is up-regula-ted, and both of them can promote the occurrence and development of hepatocellular carcinoma .
