CAJ | 학술논문

目的：观察高浓度尿酸对人近端肾小管上皮细胞氧化应激的影响，探讨其可能的作用途径。方法体外培养人近端肾小管上皮HK-2细胞，以720μmol/L的尿酸干预0、12、24、48 h后收集细胞；DCHF-DA染色检测细胞内活性氧（ROS）含量，分光光度计检测细胞上清液中的丙二醛（MDA）、总超氧化物岐化酶（T-SOD），MitoSOXTM染色观察活细胞线粒体中ROS的生成情况。结果尿酸干预12 h时细胞内总ROS含量较未干预时无明显变化，细胞上清中MDA含量、T-SOD活性亦较未干预时无明显差异（P均＞0．05）；干预24 h时总ROS含量有所升高， MDA含量上升，T-SOD活性降低，48 h时上述变化更为明显（P均＜0．05）；线粒体内ROS也在尿酸干预24 h时合成上调，48 h时升高更为显著（P＜0．05）。结论高浓度尿酸可诱导HK-2细胞氧化应激反应，线粒体ROS合成增多可能是其作用基础。
목적：관찰고농도뇨산대인근단신소관상피세포양화응격적영향，탐토기가능적작용도경。방법체외배양인근단신소관상피HK-2세포，이720μmol/L적뇨산간예0、12、24、48 h후수집세포；DCHF-DA염색검측세포내활성양（ROS）함량，분광광도계검측세포상청액중적병이철（MDA）、총초양화물기화매（T-SOD），MitoSOXTM염색관찰활세포선립체중ROS적생성정황。결과뇨산간예12 h시세포내총ROS함량교미간예시무명현변화，세포상청중MDA함량、T-SOD활성역교미간예시무명현차이（P균＞0．05）；간예24 h시총ROS함량유소승고， MDA함량상승，T-SOD활성강저，48 h시상술변화경위명현（P균＜0．05）；선립체내ROS야재뇨산간예24 h시합성상조，48 h시승고경위현저（P＜0．05）。결론고농도뇨산가유도HK-2세포양화응격반응，선립체ROS합성증다가능시기작용기출。
Objective To observe the effects of high concentrations of uric acid ( UA) on oxidative stress of human proximal renal tubular epithelial cells , and to investigate the possible pathway .Methods HK-2 cells were cultured in vitro and exposed to 720μmol/L UA for 0 h, 12 h, 24 h and 48 h.The reactive oxygen species ( ROS) production in cells was detected by DCHF-DA staining.The MDA content and T-SOD activity in the supernatants were measured by Spectro-photometer .The mitochondrial ROS production was detected by MitoSOXTM staining .Results There were no significant changes in ROS production, MDA content and T-SOD activity of HK-2 cells exposed to UA for 12 h (all P>0.05).The cell ROS production and MDA content increased , while T-SOD activity decreased when HK-2 cells were exposed to UA for 24 h.Changes were more significant at 48 h (all P<0.05).The mitochondrial ROS production was increased in HK-2 cells exposed to UA for 24 h, and it increased more when exposed to UA for 48 h (P<0.05).Conclusion High concen-trations?of UA can induce the oxidative stress in HK-2 cells through the mechanism of increasing the mitochondrial ROS production .