中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2014年
4期
571-573,576
,共4页
王慰敏%魏小华%白桂芹%付逢萍%袁永兴%李琛
王慰敏%魏小華%白桂芹%付逢萍%袁永興%李琛
왕위민%위소화%백계근%부봉평%원영흥%리침
乙型肝炎病毒%细胞凋亡%乙型肝炎病毒X抗原%滋养细胞
乙型肝炎病毒%細胞凋亡%乙型肝炎病毒X抗原%滋養細胞
을형간염병독%세포조망%을형간염병독X항원%자양세포
hepatitis B virus (HBV)%apoptosis%HBV X antigen (HBxAg)%trophoblast cells
目的:探讨乙型肝炎病毒( HBV)体外感染人绒膜滋养细胞株JEG-3后对细胞凋亡水平的影响,明确HBV感染胎盘的可能机制。方法用人HBVDNA+血清体外感染JEG-3,免疫组化和Western Blot检测细胞内HBVX抗原(HBxAg)的表达;用Annexin-V荧光素探针标记细胞表面的PS磷脂酰丝氨酸,利用流式细胞仪检测早期细胞凋亡,碘化丙啶( PI)检测中晚期细胞凋亡。结果 HBV体外感染人绒癌细胞株JEG-3后,细胞内检测到HBxAg的表达;细胞的早期凋亡率和晚期凋亡率明显降低。流式细胞仪AnnexinⅤ+PI双染方法检测细胞凋亡状态,HBV血清感染组细胞的早期凋亡水平和晚期凋亡水平均低于正常人血清对照组( t 值分别为3.27、2.01,均P<0.05),差异均有统计学意义。结论 HBV 体外感染人绒癌细胞株 JEG-3可表达HBxAg,从而抑制了细胞的早期凋亡及晚期凋亡;其是HBV感染胎盘的可能机制。
目的:探討乙型肝炎病毒( HBV)體外感染人絨膜滋養細胞株JEG-3後對細胞凋亡水平的影響,明確HBV感染胎盤的可能機製。方法用人HBVDNA+血清體外感染JEG-3,免疫組化和Western Blot檢測細胞內HBVX抗原(HBxAg)的錶達;用Annexin-V熒光素探針標記細胞錶麵的PS燐脂酰絲氨痠,利用流式細胞儀檢測早期細胞凋亡,碘化丙啶( PI)檢測中晚期細胞凋亡。結果 HBV體外感染人絨癌細胞株JEG-3後,細胞內檢測到HBxAg的錶達;細胞的早期凋亡率和晚期凋亡率明顯降低。流式細胞儀AnnexinⅤ+PI雙染方法檢測細胞凋亡狀態,HBV血清感染組細胞的早期凋亡水平和晚期凋亡水平均低于正常人血清對照組( t 值分彆為3.27、2.01,均P<0.05),差異均有統計學意義。結論 HBV 體外感染人絨癌細胞株 JEG-3可錶達HBxAg,從而抑製瞭細胞的早期凋亡及晚期凋亡;其是HBV感染胎盤的可能機製。
목적:탐토을형간염병독( HBV)체외감염인융막자양세포주JEG-3후대세포조망수평적영향,명학HBV감염태반적가능궤제。방법용인HBVDNA+혈청체외감염JEG-3,면역조화화Western Blot검측세포내HBVX항원(HBxAg)적표체;용Annexin-V형광소탐침표기세포표면적PS린지선사안산,이용류식세포의검측조기세포조망,전화병정( PI)검측중만기세포조망。결과 HBV체외감염인융암세포주JEG-3후,세포내검측도HBxAg적표체;세포적조기조망솔화만기조망솔명현강저。류식세포의AnnexinⅤ+PI쌍염방법검측세포조망상태,HBV혈청감염조세포적조기조망수평화만기조망수평균저우정상인혈청대조조( t 치분별위3.27、2.01,균P<0.05),차이균유통계학의의。결론 HBV 체외감염인융암세포주 JEG-3가표체HBxAg,종이억제료세포적조기조망급만기조망;기시HBV감염태반적가능궤제。
Objective To explore the influence of JEG-3 infected with hepatitis B virus ( HBV) in vitro on apoptosis level , so as to clarify the possible mechanism of HBV infection in placenta .Methods HBV serum infected the human chorionic trophoblast cell line ( JEG-3) in vitro.The intracellular expression of HBV X antigen ( HBxAg ) was detected by immunohistochemistry and Western Blot , and the PS phosphatidylserine on the cell surface was labeled with Annexin-V fluorescein probe .The early cell apoptosis was detected with flow cytometry and the late cell apoptosis was detected with propidium iodide .Results HBxAg could be found intracellularly after HBV infection in vitro.The early and late cell apoptotic rate reduced compared with the control group which was detected by flow cytometry Annexin Ⅴand PI staining methods (t value was 3.27 and 2.01, respectively, both P <0.05).Conclusion JEG-3 can express HBxAg after HBV infection in vitro , and thus inhibits the cell apoptosis , which may be the mechanism of HBV infection in placenta .