CAJ | 학술논문

文章比较了0.1%升汞+10%NaClO+70%乙醇；10%NaClO+70%乙醇；10%NaClO+70%乙醇+1：15洁尔灭等不同消毒方法对苜蓿种子的灭菌效果，并分析了1/2 MS培养基和滤纸+1/2 MS培养液两种萌发介质对萌发率、污染率、生长时间的影响。结果表明：0.1%升汞+10%NaClO+70%乙醇和10%NaClO+70%乙醇+1：15洁尔灭的消毒方法污染率最低，且后一种方法对操作人员相对安全；滤纸+1/2 MS培养液的发芽率和幼苗生长速度较快，污染率也较低。综合效果表明：10%NaClO+70%乙醇+1：15洁尔灭与1/2 MS培养液+滤纸的组合安全快捷，效果最佳。
문장비교료0.1%승홍+10%NaClO+70%을순；10%NaClO+70%을순；10%NaClO+70%을순+1：15길이멸등불동소독방법대목숙충자적멸균효과，병분석료1/2 MS배양기화려지+1/2 MS배양액량충맹발개질대맹발솔、오염솔、생장시간적영향。결과표명：0.1%승홍+10%NaClO+70%을순화10%NaClO+70%을순+1：15길이멸적소독방법오염솔최저，차후일충방법대조작인원상대안전；려지+1/2 MS배양액적발아솔화유묘생장속도교쾌，오염솔야교저。종합효과표명：10%NaClO+70%을순+1：15길이멸여1/2 MS배양액+려지적조합안전쾌첩，효과최가。
Comparison effect of different seed sterilization methods on alfalfa as follows:0.1%HgCl2+10%NaClO+70%ethanol;10%NaClO+70%ethanol;and 10%NaClO+70%ethanol+1:15 bromogeramine. Meanwhile,analyzing the effect of 1/2 MS culture medium and filter paper+1/2 MS solution on germination rate,pollution rate and growth time of alfalfa.The results showed that pollution rate was the lowest under both 0.1% HgCl2+10%NaClO+70% ethanol and 10%NaClO+70% ethanol +1:15 bro-mogeramine disinfection method;moreover,the later method was a relatively safe method for operating personnel. The germination rate and seedling growth speed were higher under filter paper+1/2 MS medium than 1/2 MS culture medium,while the pollution rate was lower. So,the safe and fast combination method is 10%NaClO+70%ethanol+1:15 bromogeramine+filter paper+1/2 MS solution.