作物研究
作物研究
작물연구
CROP RESEARCH
2014年
5期
467-471
,共5页
胡学芳%刘聪%肖旦望%熊兴华
鬍學芳%劉聰%肖旦望%熊興華
호학방%류총%초단망%웅흥화
甘蓝型油菜%GPAT6基因%启动子%基因克隆%表达载体构建
甘藍型油菜%GPAT6基因%啟動子%基因剋隆%錶達載體構建
감람형유채%GPAT6기인%계동자%기인극륭%표체재체구건
Brassica napus%GPAT6 gene%Promoter%Gene cloning%Expression vector construction
通过同源PCR克隆的方法，首次从甘蓝型油菜中克隆了甘油-3-磷酸酰基转移酶6（ sn-glycerol -3-phosphate acyltransferase 6，GPAT6）基因长1110 bp的启动子，并将其成功构建到植物表达载体pBI121上。重组载体可用于转化拟南芥，探究甘蓝型油菜GPAT6基因的组织表达特征。
通過同源PCR剋隆的方法，首次從甘藍型油菜中剋隆瞭甘油-3-燐痠酰基轉移酶6（ sn-glycerol -3-phosphate acyltransferase 6，GPAT6）基因長1110 bp的啟動子，併將其成功構建到植物錶達載體pBI121上。重組載體可用于轉化擬南芥，探究甘藍型油菜GPAT6基因的組織錶達特徵。
통과동원PCR극륭적방법，수차종감람형유채중극륭료감유-3-린산선기전이매6（ sn-glycerol -3-phosphate acyltransferase 6，GPAT6）기인장1110 bp적계동자，병장기성공구건도식물표체재체pBI121상。중조재체가용우전화의남개，탐구감람형유채GPAT6기인적조직표체특정。
In this study,the promoter,1 110 bp in length,of GPAT6 gene was cloned from B.napus at the first time by ho-mology-based PCR cloning ,and then it was constructed to plant expression vector pBI 121.The recombinant vector could be transformed into Arabidopsis thaliana to study the tissue expression pattern of GPAT6 gene in B.napus.
