CAJ | 학술논문

以从独角莲块茎内分离到的细菌TG116为研究对象，通过16S rRNA序列分析结合形态特征和生理生化特性对T G116进行鉴定，采用皿内涂布、平板对峙和异步培养等方法研究其抑菌特性，并对其生长曲线、最适温度、最适pH值及耐盐性等进行了系统研究。结果表明：TG116对辣椒疫霉病菌（Phytophthora capsici）等7种供试植物病原菌均有良好的掏抑制作用，表现出对植物病原真菌的广谱抗性且能使病原菌菌丝扭曲、变形等。结合形态学、生理生化指标和16S rRNA序列分析等结果，初步鉴定TG116菌株为地衣芽孢杆菌（Bacillus licheniformis）。研究结果为生物防治作物病害提供新的菌种资源，为开发和利用独角莲内生拮抗菌提供了理论依据。
이종독각련괴경내분리도적세균TG116위연구대상，통과16S rRNA서렬분석결합형태특정화생리생화특성대T G116진행감정，채용명내도포、평판대치화이보배양등방법연구기억균특성，병대기생장곡선、최괄온도、최괄pH치급내염성등진행료계통연구。결과표명：TG116대랄초역매병균（Phytophthora capsici）등7충공시식물병원균균유량호적도억제작용，표현출대식물병원진균적엄보항성차능사병원균균사뉴곡、변형등。결합형태학、생리생화지표화16S rRNA서렬분석등결과，초보감정TG116균주위지의아포간균（Bacillus licheniformis）。연구결과위생물방치작물병해제공신적균충자원，위개발화이용독각련내생길항균제공료이론의거。
The study is focused on the endophytic bacterium strain TG116 isolated from the tuber of Typhonium giganteum Engl .Morphological ,physiological and biochemical characteristics of TG116 are identified using 16S rRNA sequence analysis . Antimicrobial characteristics of TG 116 are studied using surface spread plate experiment , confront antibiotic culture experiment and differed antagonism procedure . The growth curve , optimum temperature , optimum pH value and salt tolerance of strain TG116 are systematically studied in this paper at the same time . Results show that strain TG116 display a wide range of antifungal spectrum which exhibite antibiotic activities toward all the 7 tested phytopathogens including Phytophthora capsici . The strain can cause the mycelia of fungi into distortion and deformation . According to its morphological , physiological and biochemical characteristics and 16S rRNA sequence analysis , the strain is preliminarily identified as Bacillus licheni f ormis . This study can supply new strain resource for the biological control of crop disease and can also provide evidence for exploring and utilizing antimicrobial endophytic bacteria in Typhonium giganteum Engl .