CAJ | 학술논문

目的：对A群脑膜炎球菌荚膜多糖纯化工艺的关键步骤进行分步研究，优化每一步工艺参数。方法优化十六烷基三甲基溴化铵的加入浓度、复合多糖的解离浓度和解离时间、不同厂家的苯酚、超滤和透析等工艺过程对荚膜多糖的影响。结果十六烷基三甲基溴化铵质量体积终浓度0．10％（ w/v）沉淀效果更好，纯化获得的荚膜多糖产量更高相对分子质量更大。复合多糖解离浓度越高，纯化获得的荚膜多糖相对分子质量越小。延长复合多糖解离时间有利于提高荚膜多糖产量。不同厂家的苯酚、超滤和透析等工艺对荚膜多糖的产量和分子大小没有影响。结论现行A群脑膜炎球菌荚膜多糖纯化工艺复杂，优化后的工艺提高了荚膜多糖产量，缩短了工艺用时，增加了工艺稳定性。
목적：대A군뇌막염구균협막다당순화공예적관건보취진행분보연구，우화매일보공예삼수。방법우화십륙완기삼갑기추화안적가입농도、복합다당적해리농도화해리시간、불동엄가적분분、초려화투석등공예과정대협막다당적영향。결과십륙완기삼갑기추화안질량체적종농도0．10％（ w/v）침정효과경호，순화획득적협막다당산량경고상대분자질량경대。복합다당해리농도월고，순화획득적협막다당상대분자질량월소。연장복합다당해리시간유리우제고협막다당산량。불동엄가적분분、초려화투석등공예대협막다당적산량화분자대소몰유영향。결론현행A군뇌막염구균협막다당순화공예복잡，우화후적공예제고료협막다당산량，축단료공예용시，증가료공예은정성。
Objective Key processes of purification technology for capsular polysaccharide from group A Meningococcus were studied step by step so as to optimize parameters in process .Methods Final concentration of CTAB , dissociation time and concentration of compound polysaccharide , phenol of different manufactures and techniques used in ultrafiltration and dialysis were optimized .Results To obtain a minimum KD value and higher yield of polysaccharide ,the final concen-tration of CTAB with a 0.1%(w/v) is selected.Higher concentration for dissociation of compound polysaccharide makes a smaller molecular size for capsular polysaccharide .Extended dissociation time of compound polysaccharide is beneficial to improve the yield of capsular polysaccharide .Phenol from different manufactures and techniques used in ultrafiltration and dialysis make no differences to molecular size and yield of capsular polysaccharide .Conclusion There are many steps in the conventional purification process of capsular polysaccharide from group A Meningococcus.It is by optimized parameters in process of purification that the yield of capsular polysaccharide is improved, the time is shortened and consistence of process is increased .